Тип публикации: статья из журнала
Год издания: 2023
Идентификатор DOI: 10.1017/s1479262123000552
Аннотация: <jats:title>Abstract</jats:title><jats:p>The article presents the results of the search for microsatellite or simple sequence repeat (SSR) loci with tri-, tetra-, penta- and hexanucleotide tandem repeat motifs in the draft <jats:italic>de novo</jats:italic> assembly of the Siberian fir (<jats:italic>Abies sibirica</jats:italic> LedПоказать полностьюeb.) genome and the development of convenient relatively highly and moderately polymorphic markers that can be easily genotyped even by simple gel electrophoresis. In total, 64 pairs of oligonucleotide polymerase chain reaction (PCR) primers for 32 detected microsatellite loci were designed and tested. Based on the testing results, 10 most promising polymorphic loci were selected and genotyped in eight natural populations of Siberian fir. Homologous microsatellite loci in the genome of European silver fir (<jats:italic>Abies alba</jats:italic> Mill.) were also identified by mapping Siberian fir contigs, containing SSR loci to the European silver fir genome assembly. A multiplex panel of 14 universal microsatellite loci was developed and genotyped in samples from four natural populations of <jats:italic>A. alba</jats:italic> and a small sample of eight Nordmann fir (<jats:italic>Abies nordmanniana</jats:italic> (Steven) Spach) trees.</jats:p>
Журнал: Plant Genetic Resources: Characterisation and Utilisation
Выпуск журнала: Т.21, №2
Номера страниц: 149-158
ISSN журнала: 14792621
Издатель: CABI Publishing