Тип публикации: статья из журнала
Год издания: 2022
Идентификатор DOI: 10.31857/S0026364822020052
Ключевые слова: пихта сибирская, раковые язвы, транскриптом, хвойные, Сorinectria</i&gt, Abies sibirica</i&gt, Ascomycota</i&gt, canker, conifers, Corinectria</i&gt, transcriptome, siberian fir, abies sibirica, ascomycota, corinectria
Аннотация: A new fungal disease affecting Siberian fir (<i>Abies sibirica</i>) and similar to canker in conifers caused by the fungus <i>Corinectria fuckeliana</i> has been observed in Central Siberia since 2006. Despite the similarity of symptoms related to <i>Corinectria</i> sp., the morphology and origin of this new fungal disease remainedПоказать полностьюunknown. The aim of the study was <i>de novo</i> transcriptome sequencing and annotation of the causal agent of the disease and its phylogenetic comparison with the most closely related species for further study of the pathogenicity of that species for <i>Abies sibirica</i>. A pure culture of the anticipated fungus was isolated, and its transcriptome was sequenced and annotated. <i>De novo </i>transcriptome assemblies were generated using different software (Trinity, CLC Genomic Workbench Assembler, and RNASpades), and 57% of 14 120 transcripts assembled using the CLC Genomic Workbench Assembler were completely annotated. Phylogenetic analysis based on four genes encoded 28S ribosomal RNA, α-actin, β-tubulin, and translation elongation factor 1 alpha, respectively, demonstrated that the discovered strain is very similar to <i>Corinectria fuckeliana</i> species, but likely represents a new species or very different strain confirming the assumption that the species or strain found in Siberia is a new one that has not been studied previously. Новое заболевание пихты сибирской (<i>Abies sibirica</i>), сходное с раковой язвой хвойных деревьев, вызываемой аскомицетами рода <i>Corinectria</i>, наблюдается в Центральной Сибири с 2006 г. Несмотря на частичное сходство симптомов, происхождение и морфология возбудителя этого заболевания до недавнего времени оставались неизвестными. Целью данного исследования было de novo секвенирование и аннотация транскриптома возбудителя заболевания <i>A. sibirica</i> и его филогенетическое сравнение с наиболее близкими видами. Для этого была выделена чистая культура гриба из ветвей пихты сибирской с визуальными симптомами поражения. Транскриптом сибирского штамма был секвенирован и аннотирован. De novo сборка транскриптома была осуществлена с использованием различного программного обеспечения (Trinity, CLC Genomic Workbench Assembler и RNASpades), и 57% из 14 120 транскриптов, собранных с помощью CLC Genomic Workbench Assembler, были полностью аннотированы. Филогенетический анализ, основанный на четырех генах, кодирующих 28S рибосомную РНК, α-актин, β-тубулин и фактор элонгации трансляции 1 альфа, показал, что исследуемый штамм имеет близкое сходство с видом <i>Corinectria fuckeliana</i>, но, вероятнее всего, представляет новый вид. A new fungal disease affecting Siberian fir (Abies sibirica) and similar to canker in conifers caused by the fungus Corinectria fuckeliana has been observed in Central Siberia since 2006. Despite the similarity of symptoms related to Corinectria sp., the morphology and origin of this new fungal disease remained unknown. The aim of the study was de novo transcriptome sequencing and annotation of the causal agent of the disease and its phylogenetic comparison with the most closely related species for further study of the pathogenicity of that species for Abies sibirica. A pure culture of the anticipated fungus was isolated, and its transcriptome was sequenced and annotated. De novo transcriptome assemblies were generated using different software (Trinity, CLC Genomic Workbench Assembler, and RNASpades), and 57% of 14120 transcripts assembled using the CLC Genomic Workbench Assembler were completely annotated. Phylogenetic analysis based on four genes encoded 28S ribosomal RNA, α-actin, β-tubulin, and translation elongation factor 1 alpha, respectively, demonstrated that the discovered strain is very similar to Corinectria fuckeliana species, but likely represents a new species or very different strain confirming the assumption that the species or strain found in Siberia is a new one that has not been studied previously. © 2022, Russian Academy of Sciences. All rights reserved.
Журнал: Микология и фитопатология
Выпуск журнала: Т. 56, № 2
Номера страниц: 114-126
ISSN журнала: 00263648
Место издания: Санкт-Петербург
Издатель: Российская академия наук