Год издания: 2017

Идентификатор DOI: 10.3967/bes2017.041

Ключевые слова: benzo[a]pyrene, 50-32-8, quercetin, 117-39-5, unspecific monooxygenase, 9012-80-0, 9037-52-9, 9038-14-6, Antioxidants, Aryl Hydrocarbon Hydroxylases, Benzo(a)pyrene, Carcinogens, Environmental Pollutants, Mutagens, Quercetin, RNA, Messenger

Аннотация: We studied effects of nutrient quercetin on cytochromes' P450 1A (CYP1A) activities (measured spectrofluorimetrically using 7-ethoxy-resorufin for CYP1A1 and 7-methoxy-resorufin for CYP1A2 as substrates), on mRNA levels (measured by RT-PCR), and on DNA-binding activities (evaluated by an electrophoretic mobility shift assay) of proПоказать полностьюteins regulating CYP1A expression in untreated and benzo(α)pyrene (BaP)-treated rats. Wistar rats received quercetin, BaP, or both once daily for 1-3 days. Quercetin did not influence CYP1A1 in untreated rats but inhibited BaP-mediated CYP1A induction on the transcriptional level decreasing positive input (AhR functional activity) and increasing negative input (AhRR/ARNT expression and Oct-1 and C/EBP functional activities). © 2017 The Editorial Board of Biomedical and Environmental Sciences

Журнал: Biomedical and Environmental Sciences

Выпуск журнала: Vol. 30, Is. 4

Номера страниц: 308-313

ISSN журнала: 08953988

Издатель: Elsevier Ltd

• Perepechaeva M.L. (Institute of Molecular Biology and Biophysics, Novosibirsk, Russian Federation)
• Seredina T.A. (Institute of Molecular Biology and Biophysics, Novosibirsk, Russian Federation)
• Sidorova Y.A. (Institute of Molecular Biology and Biophysics, Novosibirsk, Russian Federation)
• Pivovarova E.N. (Federal research center Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (SB RAS), Novosibirsk, Russian Federation)
• Markel A.L. (Federal research center Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (SB RAS), Novosibirsk, Russian Federation, Novosibirsk State University, Novosibirsk, Russian Federation)
• Lyakhovich V.V. (Institute of Molecular Biology and Biophysics, Novosibirsk, Russian Federation, Novosibirsk State University, Novosibirsk, Russian Federation)
• Grishanova A.Y. (Institute of Molecular Biology and Biophysics, Novosibirsk, Russian Federation)

• Scopus