Тип публикации: статья из журнала
Год издания: 2009
Идентификатор DOI: 10.1016/j.jphotobiol.2009.08.007
Ключевые слова: Bacterial bioluminescence, Xanthene dyes, Quenching, Energy transfer, Intersystem crossing, Kinetic model, dye, eosin, erythrosine, flavine mononucleotide reductase, fluorescein, luciferase, reduced nicotinamide adenine dinucleotide, xanthene derivative, article, bioluminescence, chemical model, chemical reaction kinetics, diffusion, photochemical quenching, priority journal, probability, Eosine Yellowish-(YS), Fluorescent Dyes, FMN Reductase, Kinetics, Luciferases, Luminescent Measurements, Xanthenes, Bacteria (microorganisms)
Аннотация: The paper reports the experimental results of bioluminescence quenching in the coupled enzyme system NADH:FMN-oxidoreductase-luciferase in the presence of xanthene dyes (fluorescein, eosin Y, erythrosin B) featured by the rate constants of intersystem crossing. From the spectral data and with the help of kinetic model the rate consПоказать полностьюtants of energy transfer from emitter to dye were determined (2.9 x 10(12) - 6.5 x 10(13) M(-1) s(-1)) and the emitter lifetime was estimated (1.2-2.7 ns). The calculated rate constants of energy transfer in a series of xanthene molecules are higher than the diffusion-controlled constants. The rate constants of energy transfer correlate with the probability of intersystem crossing of a dye. The combined analysis of emitter fluorescence quenching and acceptor fluorescence enhancement indicates that the significant bioluminescence intensity decrease in the presence of xanthene dyes is determined by both the quenching processes via the energy transfer and the inhibition of bioluminescent reaction. (C) 2009 Elsevier B.V. All rights reserved.
Журнал: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
Выпуск журнала: Vol. 97, Is. 3
Номера страниц: 117-122
ISSN журнала: 10111344
Место издания: LAUSANNE
Издатель: ELSEVIER SCIENCE SA