Detection of nanodiamonds in biological samples by EPR spectrometry

Описание

Тип публикации: статья из журнала

Год издания: 2017

Идентификатор DOI: 10.1134/S1607672917060138

Аннотация: In model experiments in vitro, the applicability of the EPR spectrometry method for the detection of modified nanodiamonds (MNDs) in blood and homogenates of mouse organs has been established. A characteristic signal (g = 2.003, ΔH ≈ 10 G) is observed in the samples of biomaterials containing MNDs, the intensity of which increases Показать полностьюlinearly with the concentration of nanoparticles in the range of 1.6–200 μg MNDs per 1 mL of the sample. The EPR method in biomaterials reveals the presence of intrinsic paramagnetic centers, signals from which are superimposed on the signal from the MNDs. However, the intensity of these signals is small, which makes it possible to register the MNDs using EPR spectrometry with the necessary accuracy. The data obtained open up the prospects of using the EPR method for studies of the interorgan distribution, accumulation, and elimination of MNDs during their intravenous injection into experimental animals. © 2017, Pleiades Publishing, Ltd.

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Издание

Журнал: Doklady Biochemistry and Biophysics

Выпуск журнала: Vol. 477, Is. 1

Номера страниц: 394-397

ISSN журнала: 16076729

Издатель: Maik Nauka Publishing / Springer SBM

Авторы

  • Inzhevatkin E.V. (International Scientific Center for Studies of Extreme States of Organism, Federal Research Center “Krasnoyarsk Scientific Center,” Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation, Institute of Biophysics, Federal Research Center “Krasnoyarsk Scientific Center”, Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk, Russian Federation)
  • Baron A.V. (Institute of Biophysics, Federal Research Center “Krasnoyarsk Scientific Center”, Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk, Russian Federation, Siberian Federal University, Krasnoyarsk, Russian Federation)
  • Maksimov N.G. (Institute of Biophysics, Federal Research Center “Krasnoyarsk Scientific Center”, Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk, Russian Federation, Institute of Chemistry and Chemical Technology, Federal Research Center “Krasnoyarsk Scientific Center,” Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation)
  • Volkova M.B. (Institute of Biophysics, Federal Research Center “Krasnoyarsk Scientific Center”, Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk, Russian Federation, Siberian Federal University, Krasnoyarsk, Russian Federation)
  • Puzyr A.P. (Institute of Biophysics, Federal Research Center “Krasnoyarsk Scientific Center”, Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk, Russian Federation)
  • Bondar V.S. (Institute of Biophysics, Federal Research Center “Krasnoyarsk Scientific Center”, Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk, Russian Federation)

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