Comparative investigation of polyhydroxyalkanoate scaffolds with various chemical compositions

Описание

Тип публикации: статья из журнала

Год издания: 2011

Ключевые слова: Biopolymers, Scaffolds, Tissue engineering

Аннотация: The authors have constructed and characterized a series of membranes based on resorbable polyhydroxyalkanoates of different compositions. Five PHA types have been studied: a homopolymer of 3-hydroxybutyric acid, copolymers of 3-hydroxybutyric and 4-hydroxybutyric acids, 3-hydroxybutyric and 3-hydroxyvaleric acids, 3-hydroxybutyric Показать полностьюand 3-hydroxyhexanoic acids. Scanning electron microscopy and atomicforce microscopy were used to examine the microstructure of membrane surfaces, showing that membranes based on the copolymer of 3-hydroxybutyrate and 3-hydroxyhexanoate had the roughest surface, while membranes based on the copolymer of 3-hydroxybutyrate and 3-hydroxyvalerate had the smoothest surface. The contact angle for water in air was smaller and hydrophilic properties better in the copolymer membranes than in the membranes based on the high-crystallinity homopolymer of 3-hydroxybutyric acid. The culture of mouse fibroblast cell line NIH 3,3 was used to test PHAbased membranes; results of fluorescent probes of DNA DAPI and the MTT assay show that membranes based on studied PHAs are not cytotoxic on direct contact with cells and are highly biocompatible; their adhesive properties and ability to maintain fibroblast proliferation are similar to those of polystyrene and better than those of polylactic acid membranes.

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Издание

Журнал: Cellular Transplantation and Tissue Engineering

Выпуск журнала: Vol. 6, Is. 4

Номера страниц: 54-63

ISSN журнала: 1815445X

Авторы

  • Nikolaeva E.D. (Institute of Biophisycs, SB RAS, Krasnoyarsk, Russian Federation)
  • Shishatskaya E.I. (Institute of Biophisycs, SB RAS, Krasnoyarsk, Russian Federation, Siberian Federal University, Krasnoyarsk, Russian Federation)
  • Mochalov K.E. (Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, RAS, Moscow, Russian Federation)
  • Volova T.G. (Institute of Biophisycs, SB RAS, Krasnoyarsk, Russian Federation, Siberian Federal University, Krasnoyarsk, Russian Federation)
  • Sinsky A.J. (Massachusetts Institute of Technology, Cambridge, United States)

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