Тип публикации: статья из журнала

Год издания: 2017

Идентификатор DOI: 10.1080/15321819.2017.1384390

Ключевые слова: Anti-idiotypic antibody, benzo[a]pyrene, immunology, phage display, polycyclic aromatic hydrocarbons, single-chain antibodyDNA, 9007-49-2, single chain fragment variable antibody, 334577-34-3, 334577-38-7

Аннотация: Polycyclic aromatic hydrocarbons are chemical carcinogens which could induce the development of human cancers. Anti-idiotypic antibodies against benzo[a]pyrene (BP) are perspective for human cancer immunoprophylaxis and tumor immunodiagnostic techniques. The purpose of this study was to isolate anti-idiotypic antibodies against BP Показать полностьюfrom human lymphocytes naïve phage library. The anti-idiotypic antibody, named B5, was selected. Analysis of the nucleotide and amino acid sequences B5 showed no similarity to known protein databases antibodies. B5 bound idiotypic antibodies against BP in direct and competitive ELISA. It was suggested that the B5 carried an immunological image of BP and bound the idiotypic antibodies against BP. Abbreviations: scFv: single-chain variable fragment; Ab1: idiotypic antibodies; Ab2: anti-idiotypic antibodies; CBD: cellulose binding domain; BSA: bovine serum albumin; PBS: phosphate buffer; BP-BSA: benzo[a]pyrene-BSA conjugate; Cr-BSA: chrysene-BSA conjugate; Py-BSA: pyrene-BSA conjugate; Ac-BSA: anthracene-BSA conjugate; Ba-BSA: benz[a]anthracene-BSA conjugate; PAH: polycyclic aromatic hydrocarbons; pSh: mouse idiotypic single-chain variable fragment against benzo[a]pyrene; T72: human idiotypic single-chain variable fragment against benzo[a]pyrene. © 2017 Taylor & Francis.

Журнал: Journal of Immunoassay and Immunochemistry

Выпуск журнала: Vol. 38, Is. 6

Номера страниц: 652-662

ISSN журнала: 15321819

Издатель: Taylor and Francis Inc.

• Arnst K. (Federal State Scientific Institute, Federal Research Centre Coal and Coal Chemistry, Siberian Branch of the Russian Academy of Sciences, Institute of Human Ecology, Kemerovo, Russian Federation)
• Studennikov A. (Federal State Scientific Institute, Federal Research Centre Coal and Coal Chemistry, Siberian Branch of the Russian Academy of Sciences, Institute of Human Ecology, Kemerovo, Russian Federation)
• Ustinov V. (Federal State Scientific Institute, Federal Research Centre Coal and Coal Chemistry, Siberian Branch of the Russian Academy of Sciences, Institute of Human Ecology, Kemerovo, Russian Federation)
• Glushkov A. (Federal State Scientific Institute, Federal Research Centre Coal and Coal Chemistry, Siberian Branch of the Russian Academy of Sciences, Institute of Human Ecology, Kemerovo, Russian Federation, Federal State Educational Institute of Higher Professional Education, Kemerovo State University, Kemerovo, Russian Federation)

• Scopus