Effect of tritium on luminous marine bacteria and enzyme reactions


Тип публикации: статья из журнала

Год издания: 2013

Идентификатор DOI: 10.1016/j.jenvrad.2013.01.003

Ключевые слова: Bioassay, Tritium, beta-decay, Bioluminescence, Radiotoxicity, Radiation hormesis, ?-decay, Adaptive response, Bacteria cell, Bioluminescence inhibitions, Bioluminescent assay, Chronic effects, Enzyme reaction, Enzyme systems, Exposure-time, Linear dependency, Marine bacterium, Photobacterium phosphoreum, Physiological activity, Physiological functions, Specific radioactivity, Stress recognition, Tritiated water, Bacteria, Electron microscopy, Enzymes, Ionizing radiation, Phosphorescence, Physiology, Radioactivity, bacterial DNA, tritium oxide, environmental stress, enzyme activity, marine atmosphere, microbial activity, parameterization, sensitivity analysis, adaptation, article, bacterial cell, bacterial growth, controlled study, enzyme mechanism, freeze drying, nonhuman, stress, FMN Reductase, Luminescent Measurements, Microscopy, Electron, Transmission, NAD, Photobacterium, Water Pollutants, Radioactive

Аннотация: The paper studies chronic effect of tritiated water, HTO, (0.0002-200 MBq/L) on bioluminescent assay systems: marine bacteria Photobacterium phosphoreum (intact and lyophilized) and coupled enzyme reactions. Bioluminescence intensity serves as a marker of physiological activity. Linear dependencies of bioluminescent intensity on exПоказать полностьюposure time or radioactivity were not revealed. Three successive stages in bacterial bioluminescence response to HTO were found: (1) absence of the effect, (2) activation, and (3) inhibition. They were interpreted in terms of reaction of organisms to stress-factor i.e. stress recognition, adaptive response/syndrome, and suppression of physiological function. In enzyme system, in contrast, the kinetic stages mentioned above were not revealed, but the dependence of bioluminescence intensity on HTO specific radioactivity was found. Damage of bacteria cells in HTO (100 MBq/L) was visualized by electron microscopy. Time of bioluminescence inhibition is suggested as a parameter to evaluate the bacterial sensitivity to ionizing radiation. (c) 2013 Elsevier Ltd. All rights reserved.

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Выпуск журнала: Vol. 120

Номера страниц: 19-25

ISSN журнала: 0265931X

Место издания: OXFORD



  • Selivanova M.A. (Siberian Federal University)
  • Kudryasheva N.S. (Institute of Biophysics SB RAS)
  • Mogilnaya O.A. (Institute of Biophysics SB RAS)
  • Vydryakova G.A. (Institute of Biophysics SB RAS)
  • Kuznetsov A.M. (Institute of Biophysics SB RAS)
  • Badun G.A. (Moscow State University,Chemical Department)

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