Detection of Hispidin by a Luminescent System from Basidiomycete Armillaria borealis : научное издание

Описание

Тип публикации: статья из журнала

Год издания: 2018

Идентификатор DOI: 10.1134/S1607672918030146

Аннотация: In in vitro experiments, the possibility of using a luminescent system extracted from the luminous fungus Armillaria borealis has been shown to detect and determine the concentration of hispidin. A linear dependence of the luminescent response on the content of hispidin in solutions in the concentration range of 5.4 × 10–5–1.4 × 10Показать полностью–2 µM was detected. The stability of the enzyme system and the high sensitivity of the bioluminescent reaction allows carrying out multiple measurements with the analyte detection limit of 1.3 × 10–11 g. The obtained results show the prospects of creating a rapid bioluminescent method for the analysis of medical substances or extracts from various biological objects for the presence of hispidin.

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Издание

Журнал: Doklady Biochemistry and Biophysics

Выпуск журнала: Т. 480, 1

Номера страниц: 173-176

ISSN журнала: 16076729

Место издания: Москва

Издатель: Pleiades Publishing, Ltd. (Плеадес Паблишинг, Лтд)

Авторы

  • Puzyr A.P. (Institute of Biophysics,Krasnoyarsk Research Center,Siberian Branch,Russian Academy of Sciences)
  • Medvedeva S.E. (Institute of Biophysics,Krasnoyarsk Research Center,Siberian Branch,Russian Academy of Sciences)
  • Burov A.E. (Institute of Computational Technologies,Siberian Branch,Russian Academy of Sciences)
  • Bondar V.S. (Institute of Biophysics,Krasnoyarsk Research Center,Siberian Branch,Russian Academy of Sciences)
  • Zernov Y.P. (Voevodsky Institute of Chemical Kinetics and Combustion,Siberian Branch,Russian Academy of Sciences)

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