Detection of Hispidin by a Luminescent System from Basidiomycete Armillaria borealis

Описание

Тип публикации: статья из журнала

Год издания: 2018

Идентификатор DOI: 10.1134/S1607672918030146

Аннотация: In in vitro experiments, the possibility of using a luminescent system extracted from the luminous fungus Armillaria borealis has been shown to detect and determine the concentration of hispidin. A linear dependence of the luminescent response on the content of hispidin in solutions in the concentration range of 5.4 × 10–5–1.4 × 10–2 µM was detected. The stability of the enzyme system and the high sensitivity of the bioluminescent reaction allows carrying out multiple measurements with the analyte detection limit of 1.3 × 10–11 g. The obtained results show the prospects of creating a rapid bioluminescent method for the analysis of medical substances or extracts from various biological objects for the presence of hispidin.

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Издание

Журнал: Doklady Biochemistry and Biophysics

Выпуск журнала: Т.480, 1

Номера страниц: 173-176

ISSN журнала: 16076729

Место издания: Москва

Издатель: Pleiades Publishing, Ltd. (Плеадес Паблишинг, Лтд)

Авторы

  • Puzyr A.P. (Institute of Biophysics,Krasnoyarsk Research Center,Siberian Branch,Russian Academy of Sciences)
  • Medvedeva S.E. (Institute of Biophysics,Krasnoyarsk Research Center,Siberian Branch,Russian Academy of Sciences)
  • Burov A.E. (Institute of Computational Technologies,Siberian Branch,Russian Academy of Sciences)
  • Bondar V.S. (Institute of Biophysics,Krasnoyarsk Research Center,Siberian Branch,Russian Academy of Sciences)
  • Zernov Y.P. (Voevodsky Institute of Chemical Kinetics and Combustion,Siberian Branch,Russian Academy of Sciences)

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