Nanodiamonds as an effective adsorbent for immobilization of extracellular peroxidases from luminous fungus Neonothopanus nambi to construct a phenol detection system

Описание

Тип публикации: статья из журнала

Год издания: 2018

Идентификатор DOI: 10.1080/10242422.2018.1472586

Ключевые слова: immobilization, indicator system, luminous fungus, nanodiamonds, peroxidase, β-Glucosidase

Аннотация: Modified nanodiamonds (MNDs) produced by detonation synthesis can be used as an effective adsorbent to immobilize extracellular peroxidases of the luminous basidiomycete Neonothopanus nambi. The enzymes are firmly immobilized on MND particles and exhibit catalytic activity. The indicator system (the MND–enzyme complex) reused many Показать полностьюtimes retains its ability to catalyze reaction of co-oxidation of phenol and 4-aminoantipirine in the presence of hydrogen peroxide and remains functionally active during long-term storage (for 1 month or longer) in aqueous suspensions at 4 °C. MNDs and enzymes of higher fungi can be effectively used to construct new reusable indicator systems for analytical applications such as monitoring contamination of aquatic environments by phenolic compounds.

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Издание

Журнал: Biocatalysis and Biotransformation

Номера страниц: 1-9

ISSN журнала: 10242422

Издатель: Taylor & Francis

Авторы

  • Mogilnaya O. (Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, Russia)
  • Ronzhin N. (Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, Russia)
  • Artemenko K. (Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, Russia)
  • Bondar V. (Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, Russia)

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