Antioxidant Activity of Fullerenols. Bioluminescent Monitoring in vitro

Описание

Тип публикации: доклад, тезисы доклада, статья из сборника материалов конференций

Конференция: 26th Anniversary World Congress on Biosensors (Biosensors); Gothenburg, SWEDEN; Gothenburg, SWEDEN

Год издания: 2017

Идентификатор DOI: 10.1016/j.protcy.2017.04.097

Ключевые слова: bioluminescence, enzymatic assay, toxicity sensor, antioxidant activity, fullerenol

Аннотация: Bioluminescence of isolated enzymes is a perspective phenomenon for biosensors development due to simplicity of registration of a physiological parameter - light intensity. Enzyme-based bioluminescent assay is widely used to evaluate a decrease in biochemical toxicities. Also the enzyme-based assay is used for the direct biochemicaПоказать полностьюl monitoring of oxidative toxicity. This work considers antioxidant properties of fullerenols, water-soluble polyhydroxylated derivatives of fullerenes and perspective pharmaceutical agents, in solutions of model inorganic and organic toxicants of oxidative type K-3[Fe(CN)(6)] and 1,4-benzoquinone. Two fullerenol preparations were used: C60O2-4(OH)(20-24) and mixture of two types of fullerenols C60O2-4(OH)(20-24)+C70O2-4(OH)(20-24). The enzyme-based assays showed the peculiarities of the detoxification processes: ultralow concentrations of fullerenols were active (ca 10(-17)-10(-5)g/L); no monotonic dependence of detoxification efficiency on fullerenol concentrations was observed, and detoxification of organic oxidizer solutions was more effective than that of the inorganic oxidizer. The antioxidant effects of highly diluted fullerenol solutions were attributed to hormesis phenomenon; the detoxification was concerned with stimulation of adaptive cellular response under low-dose exposures. (C) 2017 The Authors. Published by Elsevier Ltd.

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Издание

Журнал: BIOSENSORS 2016

Выпуск журнала: Vol. 27

Номера страниц: 230-231

ISSN журнала: 22120173

Место издания: AMSTERDAM

Издатель: ELSEVIER SCIENCE BV

Авторы

  • Sachkova A.S. (Natl Res Tomsk Polytech Univ, Lenin Ave 30, Tomsk 634050, Russia; SB RAS, Inst Biophys, Akademgorodok 50-50, Krasnoyarsk 660036, Russia)
  • Kovel E.S. (SB RAS, Inst Biophys, Akademgorodok 50-50, Krasnoyarsk 660036, Russia; Siberian Fed Univ, Svobodny Pr 79, Krasnoyarsk 660041, Russia)
  • Vorobeva A.A. (Natl Res Tomsk Polytech Univ, Lenin Ave 30, Tomsk 634050, Russia)
  • Kudryasheva N.S. (SB RAS, Inst Biophys, Akademgorodok 50-50, Krasnoyarsk 660036, Russia; Siberian Fed Univ, Svobodny Pr 79, Krasnoyarsk 660041, Russia)

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