Advances in the use of molecular tools in ecological and biodiversity assessment of aquatic ecosystems : материалы временных коллективов

Описание

Тип публикации: доклад, тезисы доклада, статья из сборника материалов конференций

Конференция: 19th Congress of the Iberian-Association-of-Limnology (AIL); Coimbra, PORTUGAL; Coimbra, PORTUGAL

Год издания: 2020

Идентификатор DOI: 10.23818/limn.39.27

Ключевые слова: eDNA, metabarcoding, conservation, ecological quality, species detection, rivers, lakes, thermal springs, estuaries, lagoons

Аннотация: Conservation and sustainable management of aquatic ecosystems is a priority in environmental programs worldwide. However, these aims are highly dependent on the efficiency, accuracy and cost of existent methods for the detection of keystone species and monitoring of biological communities. Rapid advances in eDNA, barcoding and metaПоказать полностьюbarcoding promoted by high-throughput sequencing technologies are generating millions of sequences in a fast way, with a promising cost reduction, and overcoming some difficulties of the traditional taxonomic approaches. This paper provides an updated broad perspective of the current developments in this dynamic field presented in the special session (SS) "The use of molecular tools in ecological and biodiversity assessment of aquatic ecosystems" of the XIX Congress of the Iberian Association of Limnology (AIL2018), held in Coimbra, Portugal. Developments presented are mainly focused on the Iberian Peninsula (Portugal and Spain, including Atlantic Macaronesian islands) but include studies in France, Germany, Finland, Russia (Siberia) and South America. The networks within which these researchers are involved are yet even broader, profiting from existing molecular facilities, and traditional taxonomic expertise, which can be viewed as a characteristic of this new research area. It was evident in the SS that the use of molecular tools is widespread, being used to study a diversity of aquatic systems, from rivers' headwaters to estuaries and coastal lagoons, and volcanic, mountain and frozen lakes to hot springs. The organisms targeted are likewise varied and include fish, macroinvertebrates, meiofauna, microalgae such as diatoms and dinoflagellates, other protists, fungi, and bacteria (cyanobacteria and other). Some studies address the whole biodiversity (i.e., all species present independently of the taxonomic group) from environmental samples of water, biofilms and preservative solution from field samples (e.g., ethanol from macroinvertebrate samples). Great advances were acknowledged in the special session, namely in the use of metabarcoding for detecting hidden biodiversity, juvenile stages, low-abundance species, non-indigenous species and toxicity potential, and ultimately for ecological monitoring of diatoms and invertebrates. Yet, several drawbacks were highlighted and need further work, which include: taxonomic gaps in the reference databases (including gaps at species level and on intraspecific variability) or absence of public databases (e.g. for meiofauna), still high sequencing costs, the need of a substantial bioinformatics effort, difficulties in establishing the amount of environmental sample necessary for a good DNA extraction and the need for testing different genetic markers to obtain accurate results.

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Издание

Журнал: LIMNETICA

Выпуск журнала: Vol. 39, Is. 1

Номера страниц: 419-440

ISSN журнала: 02138409

Место издания: MISLATA (VALENCIA)

Издатель: ASOC ESPAN LIMNOL-MISLATA

Авторы

  • Feio Maria Joao (Marine & Environm Sci Ctr MARE, Coimbra, Portugal; Univ Coimbra, Fac Sci & Technol, Dept Life Sci, Coimbra, Portugal)
  • Filipe Ana Filipa (Univ Porto, CIBIO InBio, Ctr Invest Biodiversidade & Recursos Genet, Campus Vairdo,Vila Conde, Porto, Portugal; Univ Lisbon, Inst Super Agron, Ctr Invest Biodiversidade & Recursos Genet, CIBIO InBio, Lisbon, Portugal)
  • Garcia-Raventos Aina (Univ Porto, CIBIO InBio, Ctr Invest Biodiversidade & Recursos Genet, Campus Vairdo,Vila Conde, Porto, Portugal; Univ Lisbon, Inst Super Agron, Ctr Invest Biodiversidade & Recursos Genet, CIBIO InBio, Lisbon, Portugal)
  • Ardura Alba (Univ Oviedo, Dept Funct Biol, C Julian Claveria S-N, E-33006 Oviedo, Spain)
  • Calapez Ana Raquel (Marine & Environm Sci Ctr MARE, Coimbra, Portugal; Univ Coimbra, Fac Sci & Technol, Dept Life Sci, Coimbra, Portugal; Univ Lisbon, Sch Agr, Linking Landscape Environm Agr & Food LEAF, Lisbon, Portugal)
  • Pujante Ana Maria (Labs Tecnol Levante SL, Avda Benjamin Franklin 16, Valencia 46980, Spain)
  • Mortagua Andreia (Univ Aveiro, Dept Biol & GeoBioTec GeoBioSci, GeoTechnol & GeoEngn Res Ctr, Campus Santiago, P-3810193 Aveiro, Portugal)
  • Murria Cesc (Univ Barcelona, Grup Recerca Freshwater Ecol Hydrol & Management, Avinguda Diagonal 643, E-08028 Barcelona, Spain; Univ Barcelona, Inst Recerca Biodiversitat IRBio, Dept Biol Evolut Ecol & Ciencies Ambientals, Fac Biol, Avinguda Diagonal 643, E-08028 Barcelona, Spain)
  • Diaz-de-Quijano Daniel (Siberian Fed Univ, Fac Biol & Biotechnol, Dept Aquat & Terr Ecosyst, Svobodnyy 79, Krasnoyarsk 660041, Russia)
  • Martins Filipa M. S. (Univ Porto, CIBIO InBio, Ctr Invest Biodiversidade & Recursos Genet, Campus Vairdo,Vila Conde, Porto, Portugal; Univ Porto, Dept Biol, Fac Ciencias, Porto, Portugal)
  • Duarte Sofia (Univ Minho, Ctr Mol & Environm Biol CBMA, Dept Biol, Campus Gualtar, P-4710057 Braga, Portugal)
  • Bariain Marta Sainz (Univ Cantabria, Environm Hydraul Inst, C Isabel Torres 15, Santander 39011, Spain)
  • Cordeiro Rita (Univ Acores, InBIO Lab Associado, Ctr Invest Biodiversidade & Recursos Genet, CIBIO,Fac Ciencias & Tecnol, P-9501801 Ponta Delgada, Portugal)
  • Rivera Sinziana F. (Univ Savoie Mt Blanc, INRA, CARRTEL, 75 Av Corzent, F-74200 Thonon Les Bains, France)
  • Vaisanen Leif O. S. (Univ Oulu, Dept Ecol & Genet, Stream Ecol Res Grp, Oulu, Finland)
  • Fonseca Amelia (Univ Acores, InBIO Lab Associado, Ctr Invest Biodiversidade & Recursos Genet, CIBIO,Fac Ciencias & Tecnol, P-9501801 Ponta Delgada, Portugal)
  • Goncalves Vitor (Univ Acores, InBIO Lab Associado, Ctr Invest Biodiversidade & Recursos Genet, CIBIO,Fac Ciencias & Tecnol, P-9501801 Ponta Delgada, Portugal)
  • Garcia-Vazquez Eva (Univ Oviedo, Dept Funct Biol, C Julian Claveria S-N, E-33006 Oviedo, Spain)
  • Rodriguez David Vieites (CSIC, Natl Museum Nat Sci, Spanish Natl Res Council, Calle Jose Gutierrez Abascal 2, E-28006 Madrid, Spain)
  • Ivanova Elena A. (Siberian Fed Univ, Fac Biol & Biotechnol, Dept Aquat & Terr Ecosyst, Svobodnyy 79, Krasnoyarsk 660041, Russia)
  • Costa Filipe O. (Univ Minho, Ctr Mol & Environm Biol CBMA, Dept Biol, Campus Gualtar, P-4710057 Braga, Portugal)
  • Barquin Jose (Univ Cantabria, Environm Hydraul Inst, C Isabel Torres 15, Santander 39011, Spain)
  • Rojo Veronica (Allgenetics, Edificio CICA,Campus Elvilia S-N, E-15008 La Coruna, Spain)
  • Vierna Joaquin (Allgenetics, Edificio CICA,Campus Elvilia S-N, E-15008 La Coruna, Spain)
  • Fais Maria (Univ Minho, Ctr Mol & Environm Biol CBMA, Dept Biol, Campus Gualtar, P-4710057 Braga, Portugal)
  • Suarez Marcos (Univ Oviedo, Dept Funct Biol, C Julian Claveria S-N, E-33006 Oviedo, Spain)
  • Nieminen Marko (FAUNATICA, Kutojantie 11, Espoo, Finland)
  • Hammers-Wirtz Monica (Res Inst Ecosyst Anal & Assessment, Kackertstr 10, D-52072 Aachen, Germany)
  • Kolmakova Olesia (Russian Acad Sci BI SB RAN, Biophys Inst, Siberian Branch, 50 Akad Gorodok,Str 50, Krasnoyarsk 660036, Russia)
  • Trusova Maria Y. (Russian Acad Sci BI SB RAN, Biophys Inst, Siberian Branch, 50 Akad Gorodok,Str 50, Krasnoyarsk 660036, Russia)
  • Beja Pedro (Univ Porto, CIBIO InBio, Ctr Invest Biodiversidade & Recursos Genet, Campus Vairdo,Vila Conde, Porto, Portugal; Univ Lisbon, Inst Super Agron, Ctr Invest Biodiversidade & Recursos Genet, CIBIO InBio, Lisbon, Portugal)
  • Gonzalez Raquel (Labs Tecnol Levante SL, Avda Benjamin Franklin 16, Valencia 46980, Spain)
  • Planes Serge (Univ Perpignan, EPHE UPVD CNRS, 52 Ave Paul Alduy, F-66860 Perpignan, France; CRIOBE, Lab Excellence Corail, BP 1013, Moorea, French Polynesi, FranceArticleProceedings Paper)
  • Almeida Salome F. P. (Univ Aveiro, Dept Biol & GeoBioTec GeoBioSci, GeoTechnol & GeoEngn Res Ctr, Campus Santiago, P-3810193 Aveiro, Portugal)

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