Тип публикации: статья из журнала
Год издания: 2015
Идентификатор DOI: 10.1134/S1022795415120108
Ключевые слова: mitochondrial DNA, next generation sequencing, markers, phylogeography, Pinus sylvestris, Scots pine, DNA minisatellite, genomic DNA, allele, Article, DNA marker, DNA sequence, genotype, haplotype, intron, mitochondrial gene, mutation rate, nonhuman, polymerase chain reaction, population differentiation, population genetic structure, priority journal, single nucleotide polymorphism
Аннотация: Fragments of genomic DNA of Scots pine (Pinus sylvestris L.) homologous to the mitochondrial DNA (mtDNA) contigs of Norway spruce (Picea abies (L.) Karst.) and loblolly pine (Pinus taeda L.) were resequenced in a sample of the Scots pine trees of European, Siberian, Mongolian, and Caucasian origin in order to develop mtDNA markers.Показать полностьюFlanking non-coding regions of some mitochondrial genes were also investigated and resequenced. Five single nucleotide polymorphisms (SNPs) and a single minisatellite locus were identified. Caucasian samples differed from the rest by three SNPs. Two SNPs have been linked to an early described marker in the first intron of the nad7 gene, and all together revealed three haplotypes in European populations. No variable SNPs were found in the Siberian and Mongolian populations. The minisatellite locus contained 41 alleles across European, Siberian, and Mongolian populations, but, this locus demonstrated a weak population differentiation (F (ST) = 5.8), probably due to its high mutation rate.
Журнал: RUSSIAN JOURNAL OF GENETICS
Выпуск журнала: Vol. 51, Is. 12
Номера страниц: 1199-1203
ISSN журнала: 10227954
Место издания: NEW YORK
Издатель: MAIK NAUKA/INTERPERIODICA/SPRINGER