Study of photodissociation parameters of carboxyhemoglobin

Описание

Тип публикации: статья из журнала

Год издания: 2008

Идентификатор DOI: 10.1070/QE2008v038n07ABEH013558

Ключевые слова: carboxyhemoglobin, photodissociation, natural photolysis conditions, Carboxyhemoglobin, Natural photolysis conditions, Photodissociation, Animal cell culture, Dissociation, Irradiation, Photolysis, Porphyrins, Solutions, At wavelengths, Average powers, Buffer solutions, Carboxyhemoglobin, Experimental conditions, Flash photolysises, Human bloods, Irradiation intensities, Model experiments, Natural photolysis conditions, Photoinduced dissociations, Q transitions, Radiation wavelengths, Saturation degrees, Set-ups, Photodissociation

Аннотация: The general properties of photodissociation of carboxyhemoglobin (HbCO) in buffer solutions of whole human blood are studied by the flash photolysis method on a setup with intersecting beams. It is shown that the efficiency of photoinduced dissociation of the HbCO complex virtually linearly depends on the photolytic irradiation intПоказать полностьюensity for the average power density not exceeding 45 mW cm(-2). The general dissociation of the HbCO complex in native conditions occurs in a narrower range of values of the saturation degree than in model experiments with the hemoglobin solution. The dependence of the pulse photolysis efficiency of HbCO on the photolytic radiation wavelength in the range from 550 to 585 nm has a broad bell shape. The efficiency maximum corresponds to the electronic Q transition (porphyrin pi - pi* absorption) in HbCO at a wavelength of 570 nm. No dissociation of the complex was observed under given experimental conditions upon irradiation of solutions by photolytic radiation at wavelengths above 585 nm.

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Издание

Журнал: QUANTUM ELECTRONICS

Выпуск журнала: Vol. 38, Is. 7

Номера страниц: 695-701

ISSN журнала: 10637818

Место издания: LETCHWORTH

Издатель: TURPION LTD

Авторы

  • Kuz'min V.V. (Krasnoyarsk State Univ, Siberian Fed Univ, Inst Nat Sci & Humanities, Krasnoyarsk 660041, Russia)
  • Salmin V.V. (Krasnoyarsk State Univ, Siberian Fed Univ, Inst Nat Sci & Humanities, Krasnoyarsk 660041, Russia)
  • Salmina A.B. (Krasnoyarsk State Med Acad, Krasnoyarsk 660022, Russia)
  • Provorov A.S. (Krasnoyarsk State Univ, Siberian Fed Univ, Inst Nat Sci & Humanities, Krasnoyarsk 660041, Russia)

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