Physiological and biochemical characteristics and capacity for polyhydroxyalkanoates synthesis in a glucose-utilizing strain of hydrogen-oxidizing bacteria, Ralstonia eutropha B8562

Описание

Тип публикации: статья из журнала

Год издания: 2005

Идентификатор DOI: 10.1007/s11021-005-0124-6

Ключевые слова: hydrogen-oxidizing bacteria; glucose-utilizing strain; polyhydroxyalkanoates; synthesis

Аннотация: The physiological, biochemical, genetic, and cultural characteristics of the glucose-utilizing mutant strain Ralstonia eutropha B8562 were investigated in comparison with the parent strain R. eutropha B5786. The morphological, cultural, and biochemical characteristics of strain R. eutropha B8562 were similar to those of strain R. eПоказать полностьюutropha B5786. Genetic analysis revealed differences between the 16S rRNA gene sequences of these strains. The growth characteristics of the mutant using glucose as the sole carbon and energy source were comparable with those of the parent strain grown on fructose. Strain B8562 was characterized by high yields of polyhydroxyalkanoate (PHA) from different carbon sources (CO2, fructose, and glucose). In batch culture with glucose under nitrogen limitation, PHA accumulation reached 90% of dry weight. In PHA, beta-hydroxybutyrate was predominant (over 99 mol %); beta-hydroxyvalerate (0.25-0.72 mol %) and beta-hydroxyhexanoate (0.008-1.5 mol %) were present as minor components. The strain has prospects as a PHA producer on glucose-containing media.

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Издание

Журнал: MICROBIOLOGY

Выпуск журнала: Vol. 74, Is. 6

Номера страниц: 684-689

ISSN журнала: 00262617

Место издания: NEW YORK

Издатель: MAIK NAUKA/INTERPERIODICA

Авторы

  • Volova T.G. (Institute of Biophysics,Siberian Division,Russian Academy of Sciences)
  • Dolgopolova Yu.B. (Institute of Biophysics,Siberian Division,Russian Academy of Sciences)
  • Trusova M.Yu. (Institute of Biophysics,Siberian Division,Russian Academy of Sciences)
  • Kalacheva G.S. (Institute of Biophysics,Siberian Division,Russian Academy of Sciences)
  • Kozhevnikov I.V. (Krasnoyarsk State University)
  • Aref'eva Yu.V. (Krasnoyarsk State University)

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