Affine magnetic sorbents supported on coal ash microspheres for recombinant protein isolation

Описание

Тип публикации: статья из журнала

Год издания: 2009

Идентификатор DOI: 10.1134/S0003683809020173

Ключевые слова: Clytia gregaria, Obelia longissima

Аннотация: The results of the development and utilization of an affine magnetic sorbent with Ni(2+) ions immobilized on coal ash microspheres are reported. The applicability of the material in the isolation of Histag proteins is demonstrated by examples of the recombinant green fluorescent protein from Clytia gregaria and the Ca(2+) regulatedПоказать полностьюphotoprotein obelin from Obelia longissima. The specific sorption capacity of the sorbent was 2-7 mg/cm(3) for medium-size proteins (20-30 kDa). The particles are suitable for chromatography with the presence of chaotropic agents and EDTA. They are easy to manipulate as isolation of a target protein takes 30-35 min. On the one hand, the elevated affinity of the sorbent to proteins rich in native histidines may result in a high degree of irreversible sorption; on the other hand, it allows isolation of such proteins without the introduction of artificial polyhistidine fragments.

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Издание

Журнал: APPLIED BIOCHEMISTRY AND MICROBIOLOGY

Выпуск журнала: Vol. 45, Is. 2

Номера страниц: 215-220

ISSN журнала: 00036838

Место издания: NEW YORK

Издатель: MAIK NAUKA/INTERPERIODICA/SPRINGER

Авторы

  • Frank L.A. (Institute of Biophysics, Siberian Branch,Russian Academy of Sciences)
  • Borisova V.V. (Institute of Biophysics, Siberian Branch,Russian Academy of Sciences)
  • Gitelson I.I. (Siberian Federal University)
  • Vereshchagina T.A. (Institute of Chemistry and Chemical Technology,Siberian Branch,Russian Academy of Sciences)
  • Fomenko E.V. (Institute of Chemistry and Chemical Technology,Siberian Branch,Russian Academy of Sciences)
  • Anshits A.G. (Siberian Federal University)

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