Protein-based fluorescent bioassay for low-dose gamma radiation exposures

Описание

Тип публикации: статья из журнала

Год издания: 2018

Идентификатор DOI: 10.1007/s00216-018-1282-5

Ключевые слова: Bioassay, Enzymes, Fluorescence/luminescence, Fluorescent protein, Gamma radiation, Radiotoxicity

Аннотация: The study suggests an application of a coelenteramide-containing fluorescent protein (CLM-CFP) as a simplest bioassay for gamma radiation exposures. “Discharged obelin,” a product of the bioluminescence reaction of the marine coelenterate Obelia longissima, was used as a representative of the CLM-CFP group. The bioassay is based onПоказать полностьюa simple enzymatic reaction—photochemical proton transfer in the coelenteramide-apoprotein complex. Components of this reaction differ in fluorescence color, providing, by this, an evaluation of the proton transfer efficiency in the photochemical process. This efficiency depends on the microenvironment of the coelenteramide within the protein complex, and, hence, can evaluate a destructive ability of gamma radiation. The CLM-CFP samples were exposed to gamma radiation (137Cs, 2 mGy/h) for 7 and 16 days at 20 °C and 5 °C, respectively. As a result, two fluorescence characteristics (overall fluorescence intensity and contributions of color components to the fluorescence spectra) were identified as bioassay parameters. Both parameters demonstrated high sensitivity of the CLM-CFP-based bioassay to the low-dose gamma radiation exposure (up to 100 mGy). Higher temperature (20 °C) enhanced the response of CLM-CFP to gamma radiation. This new bioassay can provide fluorescent multicolor assessment of protein destruction in cells and physiological liquids under exposure to low doses of gamma radiation. [Figure not available: see fulltext.]. © 2018, Springer-Verlag GmbH Germany, part of Springer Nature.

Ссылки на полный текст

Издание

Журнал: Analytical and Bioanalytical Chemistry

Авторы

  • Petrova A.S. (Krasnoyarsk State Agrarian University, Mira Avenue 90, Krasnoyarsk, Russian Federation)
  • Lukonina A.A. (Siberian Federal University, Svobodnyy Ave 79, Krasnoyarsk, Russian Federation)
  • Dementyev D.V. (Institute of Biophysics SB RAS, FRC KSC SB RAS, Krasnoyarsk, Russian Federation)
  • Ya. Bolsunovsky A. (Institute of Biophysics SB RAS, FRC KSC SB RAS, Krasnoyarsk, Russian Federation)
  • Popov A.V. (Department of Radiology, University of Pennsylvania, 3401 N Broad St., Philadelphia, PA, United States)
  • Kudryasheva N.S. (Siberian Federal University, Svobodnyy Ave 79, Krasnoyarsk, Russian Federation, Institute of Biophysics SB RAS, FRC KSC SB RAS, Krasnoyarsk, Russian Federation)

Вхождение в базы данных