Тип публикации: статья из журнала
Год издания: 2013
Идентификатор DOI: 10.1016/j.resmic.2012.10.008
Ключевые слова: Poly(3-hydroxybutyrate), Granules, PHA cycle, Ralstonia eutropha, poly(3 hydroxybutyric acid), article, bacterial growth, bacterial strain, bacterium culture, cell division, cell granule, cell size, controlled study, Cupriavidus necator, electron microscopy, nonhuman, polymer production, priority journal, Cytoplasmic Granules, Hydroxybutyrates, Microscopy, Electron, Transmission, Polyesters, Bacteria (microorganisms)
Аннотация: Microbial polyhydroxyalkanoates (PHAs), because of their well studied complex physiology and commercial potential, are vehicles for carbon and potential storage reduction for many microbial species. Even with the wealth of studies about microbial PHAs in the scientific literature, polymer accumulation and degradation are still not Показать полностьюcomprehensively understood. Poly(3-hydroxybutyrate) (P3HB) granule formation and polymer mobility were studied here in the bacterium Ralstonia eutropha strain B5786 in autotrophic cultures. Electron microscopy studies revealed decreasing cell size concomitant with enlargement of size and number of intracellular granules, and inhibition of cell division during intracellular polymer production. Activities of key P3HB biosynthetic enzymes demonstrated correlations with each other during polymer accumulation, suggesting an intricately regulated P3HB cycle in autotrophically grown R. eutropha cells. (C) 2012 Published by Elsevier Masson SAS on behalf of Institut Pasteur.
Журнал: RESEARCH IN MICROBIOLOGY
Выпуск журнала: Vol. 164, Is. 2
Номера страниц: 164-171
ISSN журнала: 09232508
Место издания: AMSTERDAM
Издатель: ELSEVIER SCIENCE BV