Тип публикации: статья из журнала
Год издания: 2015
Идентификатор DOI: 10.2174/1386207318666150917100011
Ключевые слова: Luciferase, luciferin, photoprotein, bioluminescence, mutagenesis, luciferase-based assay, bioimaging, reporter assay
Аннотация: Bioluminescent proteins have been intensively used as high sensitive reporters in all kinds of binding assays (immuno-, nucleic acid hybridization assays, etc.) and in bioimaging. But natural luciferases do not always meet the requirements set for them as the assay reporters: thermostabitity, definite bioluminescence spectral and kПоказать полностьюinetics characteristics, stability to chemical modifications, etc. Luciferases with different appropriate characteristics as well as various luciferin derivatives were obtained using mutagenesis and chemical synthesis. Thanks to rigorous efforts of many researchers bioluminescence-based analytical techniques offer a great potential for solving analytical tasks in the field of biotechnology, biomedicine, pharmacology, etc.
Журнал: COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING
Выпуск журнала: Vol. 18, Is. 10
Номера страниц: 919-929
ISSN журнала: 13862073
Место издания: SHARJAH
Издатель: BENTHAM SCIENCE PUBL LTD