Immunohistochemical study for the expression of Bcl-2 family proteins in walker 256 carcinosarcoma cells under the influence of cytostatic drugs

Описание

Тип публикации: статья из журнала

Год издания: 2009

Идентификатор DOI: 10.1007/s10517-010-0787-5

Ключевые слова: Apoptosis, Cyclophosphamide, Melatonin, Proteins of the Bcl-2 family, Walker 256 carcinosarcoma, cytostatic agent, protein BAD, protein Bax, protein bcl 2, animal experiment, article, cancer combination chemotherapy, cancer regression, cancer transplantation, carcinogenesis, controlled study, immunohistochemistry, male, monotherapy, nonhuman, protein expression, protein family, rat, Walker carcinoma, Wistar rat, Animals, Antineoplastic Agents, Alkylating, Antioxidants, bcl-2-Associated X Protein, bcl-Associated Death Protein, Carcinoma 256, Walker, Cytostatic Agents, Humans, Neoplasm Transplantation, Proto-Oncogene Proteins c-bcl-2, Rats, Rats, Wistar, Tumor Cells, Cultured, Rattus norvegicus

Аннотация: Immunohistochemical study was performed to evaluate the expression of Bcl-2 family proteins (Bcl-2, Bax, and Bad) in Walker 256 carcinosarcoma cells after implantation into the thigh muscle of male Wistar rats (106 cells). The experiment was conducted under conditions of spontaneous tumor development and individual or combined treaПоказать полностьюtment with melatonin and cyclophosphamide. The use of melatonin as monotherapy or in combination with cyclophosphamide was followed by a signifi cant decrease in Bcl-2 expression in carcinosarcoma cells. The Bcl-2/Bax and Bcl-2/Bad ratio was signifi cantly reduced under these conditions (particularly after combined treatment with cytostatic drugs). These changes were accompanied by a signifi cant (by 93.61%) decrease in the volume of transplantable tumor on day 14. Daily treatment with melatonin was accompanied by signifi cant changes in the structure of Walker 256 carcinosarcoma. It was manifested in the formation of connective tissue septa and pseudofollicles. © 2009 Springer Science+Business Media, Inc.

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Издание

Журнал: Bulletin of Experimental Biology and Medicine

Выпуск журнала: Vol. 148, Is. 4

Номера страниц: 650-655

Персоны

  • Ovsjanko E.V. (Novosibirsk State Medical University, Federal Agency for Health Care and Social Development, Novosibirsk, Russian Federation)
  • Lushnikova E.L. (Institute of Regional Pathology and Pathomorphology, Siberian Division, Russian Academy of Medical Sciences, Novosibirsk, Russian Federation)
  • Larionov P.M. (E. N. Meshalkin Novosibirsk Institute for Pathology of Blood Circulation, Russian Medical Technologies, Novosibirsk, Russian Federation)
  • Arkhipov S.A. (Novosibirsk State Medical University, Federal Agency for Health Care and Social Development, Novosibirsk, Russian Federation)
  • Nepomnyashchikh L.M. (Institute of Regional Pathology and Pathomorphology, Siberian Division, Russian Academy of Medical Sciences, Novosibirsk, Russian Federation)
  • Efremov A.V. (Novosibirsk State Medical University, Federal Agency for Health Care and Social Development, Novosibirsk, Russian Federation)
  • Ovsjanko Y.U. (Novosibirsk State Medical University, Federal Agency for Health Care and Social Development, Novosibirsk, Russian Federation)

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