Тип публикации: статья из журнала

Год издания: 2009

Идентификатор DOI: 10.1134/S1995078009090158

Ключевые слова: Colloidal solutions, Concentration of, Electronic microscopy, Electroporation, Intracellular spaces, Nanoparticle solutions, Oligonucleotide conjugates, Phagosomes, TiO, Titanium dioxide nanoparticles, Nanoparticles, Nucleic acids, Oligonucleotides, Organic polymers, Titanium, Titanium dioxide, Cell culture

Аннотация: In this study we examine the possibility that TiO2 nanoparticles and their conjugates can penetrate into cultivated cells without any special transfection procedures. Oligonucleotides and their derivates were conjugated with the TiO2 nanoparticles, which were obtained as colloidal solutions at a concentration of TiO2 0.3M by TiCl4 Показать полностьюhydrolysis. The electronic microscopy of various cell cultures (KCT, Vero, and MDCK) treated with nanoparticle solutions (20 ?g/?l) showed that nanoparticles could enter the cells and accumulate in the vacuoles and phagosomes and form inclusions in cytoplasm. Thus, we demonstrated the penetration of TiO2 nanoparticles and their oligonucleotide conjugates into intracellular space without any auxiliary operations. Most other researches used electroporation techniques for similar purposes [1, 2, 5]. © 2009 Pleiades Publishing, Ltd.

Журнал: Nanotechnologies in Russia

Выпуск журнала: Vol. 4, Is. 9-10

Номера страниц: 732-735

• Zarytova V.F. (Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, pr. Akademika Lavrent'eva 8, Novosibirsk 630090, Russian Federation)
• Zinov'ev V.V. (The Vektor State Research Center of Virology and Biotechnology, Federal Service for Supervision in the Sphere of Protecting Consumer Rights and Human Wellbeing, ABK, korp. 12, Kol'tsovo, Novosibirsk oblast 630559, Russian Federation)
• Ismagilov Z.R. (Institute of Catalysis, Siberian Branch, Russian Academy of Sciences, pr. Akademika Lavrent'eva 5, Novosibirsk 630090, Russian Federation)
• Levina A.S. (Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, pr. Akademika Lavrent'eva 8, Novosibirsk 630090, Russian Federation)
• Repkova M.N. (Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, pr. Akademika Lavrent'eva 8, Novosibirsk 630090, Russian Federation)
• Shikina N.V. (Institute of Catalysis, Siberian Branch, Russian Academy of Sciences, pr. Akademika Lavrent'eva 5, Novosibirsk 630090, Russian Federation)
• Evdokimov A.A. (The Vektor State Research Center of Virology and Biotechnology, Federal Service for Supervision in the Sphere of Protecting Consumer Rights and Human Wellbeing, ABK, korp. 12, Kol'tsovo, Novosibirsk oblast 630559, Russian Federation)
• Belanov E.F. (The Vektor State Research Center of Virology and Biotechnology, Federal Service for Supervision in the Sphere of Protecting Consumer Rights and Human Wellbeing, ABK, korp. 12, Kol'tsovo, Novosibirsk oblast 630559, Russian Federation)
• Balakhnin S.M. (The Vektor State Research Center of Virology and Biotechnology, Federal Service for Supervision in the Sphere of Protecting Consumer Rights and Human Wellbeing, ABK, korp. 12, Kol'tsovo, Novosibirsk oblast 630559, Russian Federation)
• Serova O.A. (The Vektor State Research Center of Virology and Biotechnology, Federal Service for Supervision in the Sphere of Protecting Consumer Rights and Human Wellbeing, ABK, korp. 12, Kol'tsovo, Novosibirsk oblast 630559, Russian Federation)
• Baiborodin S.I. (State Educational Institution of Higher Professional Education, Novosibirsk State University Rosobrazovaniya, Russian Federation, ul. Pirogova 2, Novosibirsk 630090, Russian Federation)
• Malygin E.G. (The Vektor State Research Center of Virology and Biotechnology, Federal Service for Supervision in the Sphere of Protecting Consumer Rights and Human Wellbeing, ABK, korp. 12, Kol'tsovo, Novosibirsk oblast 630559, Russian Federation)
• Zagrebel'nyi S.N. (State Educational Institution of Higher Professional Education, Novosibirsk State University Rosobrazovaniya, Russian Federation, ul. Pirogova 2, Novosibirsk 630090, Russian Federation)

• Scopus