Тип публикации: статья из журнала

Год издания: 2017

Идентификатор DOI: 10.1111/php.12648

Аннотация: Ca2+-regulated photoprotein obelin was genetically fused with a minimum-sized core streptavidin. Hybrid protein (SAV–OL) was produced by bacterial expression and applied as a specific bioluminescent probe in diverse solid-phase assays. The obtained results clearly demonstrate specific activity of each domain indicating its proper fПоказать полностьюolding with favorable space orientation. SAV–OL has been shown to be a much more sensitive label than the chemical conjugate of a full-length streptavidin with obelin. © 2016 The American Society of Photobiology

Журнал: Photochemistry and Photobiology

Выпуск журнала: Vol. 93, Is. 2

Номера страниц: 548-552

• Bashmakova E.E. (Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation, Siberian Federal University, Krasnoyarsk, Russian Federation)
• Krasitskaya V.V. (Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation)
• Kudryavtsev A.N. (Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation)
• Grigorenko V.G. (Chemistry Department, Lomonosov Moscow State University, Moscow, Russian Federation)
• Frank L.A. (Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation, Siberian Federal University, Krasnoyarsk, Russian Federation)

• Scopus
• Web of Science Core Collection
• Ядро РИНЦ (eLIBRARY.RU)