Тип публикации: статья из журнала
Год издания: 2020
Идентификатор DOI: 10.1134/S1607672920010135
Ключевые слова: basidiomycete Neonothopanus nambi, extracellular oxidases, FAD, gel-filtration chromatography, phenol, veratryl alcohol, ?-glucosidase, beta-glucosidase
Аннотация: Using the original technique of treating biomass with β-glucosidase, a pool of extracellular fungal enzymes was obtained for the first time from the mycelium of basidiomycete Neonothopanus nambi. Two protein fractions containing enzymes with oxidase activity were isolated from the extract by gel-filtration chromatography and convenПоказать полностьюtionally called F1 and F2. Enzyme F1 has a native molecular weight of 80–85 kDa and does not contain chromophore components; however, it catalyzes the oxidation of veratryl alcohol with Km = 0.52 mM. Probably, this enzyme is an alcohol oxidase. Enzyme F2 with a native molecular weight of approximately 60 kDa is a FAD-containing protein. It catalyzes the cooxidation of phenol with 4-aminoantipyrine without the addition of exogenous hydrogen peroxide, which distinguishes it from the known peroxidases. It was assumed that this enzyme may be a mixed-function oxidase. F2 oxidase has Km value 0.27 mM for phenol. The temperature optimums for oxidases F1 and F2 are 22–35 and 55–70°C, and pH optimums are 6 and 5, respectively. © 2020, Pleiades Publishing, Ltd.
Журнал: Doklady Biochemistry and Biophysics
Выпуск журнала: Vol. 490, Is. 1
Номера страниц: 38-42
ISSN журнала: 16076729
Издатель: Pleiades Publishing