The effect of individual genetic heterozygosity on general homeostasis, heterosis and resilience in Siberian larch (Larix sibirica Ledeb.) using dendrochronology and microsatellite loci genotyping


Тип публикации: статья из журнала

Год издания: 2016

Идентификатор DOI: 10.1016/j.dendro.2016.02.005

Ключевые слова: Dendrochronology, Tree ring width, Radial growth, Individual heterozygosity, Microsatellite markers, Heterosis, Homeostasis, Climate change, Environmental stress, coniferous tree, DNA, ecosystem resilience, genetic analysis, genetic marker, growth rate, heterozygosity, tree ring, Siberia, Larix, Larix sibirica

Аннотация: The genetic mechanisms underlying the relationship of individual heterozygosity (IndHet) with heterosis and homeostasis are not fully understood. Such an understanding, however, would have enormous value as it could be used to identify trees better adapted to environmental stress. Dendrochronology data, in particular the individualПоказать полностьюaverage radial increment growth of wood measured as the average tree ring width (AvTRW) and the variance of tree ring width (VarTRW) were used as proxies for heterosis (growth rate measured as AvTRW) and homeostasis (stability of the radial growth of individual trees measured as VarTRW), respectively. These traits were then used to test the hypothesis that IndHet can be used to predict heterosis and homeostasis of individual trees. Wood core and needle samples were collected from 100 trees of Siberian larch (Larix sibirica Ledeb.) across two populations located in Eastern Siberia. DNA samples were obtained from the needles of each individual tree and genotyped for eight highly polymorphic microsatellite loci. Then mean IndHet calculated based on the genotypes of eight loci for each tree was correlated with the statistical characteristics of the measured radial growth (AvTRW and VarTRW) and the individual standardized chronologies. The analysis did not reveal significant relationships between the studied parameters. In order to account for the strong dependence of the radial growth on tree age the age curves were examined. An original approach was employed to sort trees into groups based on the distance between these age curves. No relationship was found between these groups and the groups formed based on heterozygosity. However, further work with more genetic markers and increased sample sizes is needed to test this novel approach for estimating heterosis and homeostasis. (C) 2016 Elsevier GmbH. All rights reserved.

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Выпуск журнала: Vol. 38

Номера страниц: 26-37

ISSN журнала: 11257865

Место издания: JENA



  • Babushkina E.A. (Khakasia Technical Institute,Siberian Federal University)
  • Grachev A.M. (Institute of Geography,Russian Academy of Sciences)
  • Belokopytova L.V. (Khakasia Technical Institute,Siberian Federal University)
  • Kostyakova T.V. (Khakasia Technical Institute,Siberian Federal University)
  • Vaganov E.A. (Siberian Federal University)
  • Oreshkova N.V. (V.N. Sukachev Institute of Forest,Siberian Branch,Russian Academy of Sciences)
  • Krutovsky K.V. (Siberian Federal University; Georg-August-University of Göttingen; N.I. Vavilov Institute of General Genetics, Russian Academy of Sciences; Texas AandM University)