Тип публикации: статья из журнала
Год издания: 2012
Идентификатор DOI: 10.1016/j.jphotobiol.2012.09.020
Ключевые слова: Metal salts, Humic substances, Toxicity, Bioluminescent assay, Enzyme reaction rates, Photobacterium phosphoreum, chromium chloride, cobalt chloride, copper sulfate, ferrocyanide, lead nitrate, article, bacterial cell, bioassay, bioluminescence, cell surface, chemical reaction, constants and coefficients, controlled study, detoxification, enzyme mechanism, humic substance, nonhuman, pollutant, priority journal, ultrastructure, Biological Assay, Environmental Pollutants, Luminescent Measurements, Metals, Photobacterium, Salts, Solutions, Bacteria (microorganisms)
Аннотация: The paper considers effects of humic substances (HS), as natural attenuators of toxicity, on solutions of model inorganic pollutants, metal salts - Pb(NO3)(2), CoCl2, CuSO4, Eu(NO3)(3), CrCl3, and K-3[Fe(CN)(6)] Luminous bacteria Photobacterium phosphoreum and bioluminescent system of coupled enzymatic reactions were used as bioassПоказать полностьюays to monitor toxicity of salt solutions. The ability of HS to decrease or increase toxicity was demonstrated. Detoxifying concentrations of HS were determined; detoxification coefficients were calculated at different times of exposure of salt solutions to HS. To study the combined effects of HS and salts on bioluminescent assay systems, the rates of biochemical reactions and bacterial ultrastructure were analyzed. The detoxifying effects were explained by: (1) decrease of free metal content in water solutions under metal-HS binding; (2) increase of biochemical reaction rates in a bioluminescent assay system under HS effect; (3) enhancement of mucous layers on cell surface as a response to unfavorable impact of toxicants. Detoxifying mechanisms (2) and (3) reveal the active role of bioassay systems in detoxification processes. (C) 2012 Elsevier B.V. All rights reserved.
Журнал: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
Выпуск журнала: Vol. 117
Номера страниц: 164-170
ISSN журнала: 10111344
Место издания: LAUSANNE
Издатель: ELSEVIER SCIENCE SA