Polymer Films of Poly-3-hydroxybutyrate Synthesized by Cupriavidus necator from Different Carbon Sources : научное издание

Описание

Тип публикации: статья из журнала

Год издания: 2020

Идентификатор DOI: 10.1007/s10924-020-01924-3

Ключевые слова: degradable p(3hb), various carbon substrates, films, structure, properties, nih 3t3 fibroblasts

Аннотация: Films were prepared from 2% solutions of biodegradable poly-3-hydroxybutyrate [P(3HB)] and investigated. The polymer was synthesized by theCupriavidus necatorB-10646 bacterium cultivated using various carbon sources (glucose and glycerol of different degrees of purity, containing 0.3 to 17.93% impurities). Glycerol as the substrateПоказать полностьюinfluenced molecular-weight properties and crystallinity of the polymer without affecting its temperature characteristics. The P(3HB) specimens synthesized from glycerol had reduced M-w(300-400 kDa) and degree of crystallinity (50-55%) compared to the specimens synthesized from glucose (860 kDa and 76%, respectively). The low-crystallinity P(3HB) specimens, regardless of the degree of purity of glycerol, produced a beneficial effect on the properties of polymer films, which had a better developed folded surface and increased hydrophilicity. The values of the highest roughness (R-a) of the films synthesized from glycerol were 1.8 to 4.0 times lower and the water angles 1.4-1.6 times smaller compared to the films synthesized from glucose (71.75 nm and 87.4 degrees, respectively). Those films performed better as cell scaffolds: the number of viable NIH fibroblasts was 1.7-1.9 times higher than on polystyrene (control) or films of P(3HB) synthesized from glucose.

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Издание

Журнал: JOURNAL OF POLYMERS AND THE ENVIRONMENT

Выпуск журнала: Vol. 29, Is. 3

ISSN журнала: 15662543

Место издания: NEW YORK

Издатель: SPRINGER

Авторы

  • Shishatskaya Ekaterina (Siberian Fed Univ, 79 Svobodnyi Av, Krasnoyarsk 660041, Russia; RAS, Krasnoyarsk Sci Ctr SB, Fed Res Ctr, Inst Biophys, Krasnoyarsk, Russia)
  • Nemtsev Ivan (RAS, Krasnoyarsk Sci Ctr SB, Fed Res Ctr, LV Kirenskii Inst Phys, Krasnoyarsk, Russia)
  • Lukyanenko Anna (RAS, Krasnoyarsk Sci Ctr SB, Fed Res Ctr, LV Kirenskii Inst Phys, Krasnoyarsk, Russia)
  • Vasiliev Alexander (Siberian Fed Univ, 79 Svobodnyi Av, Krasnoyarsk 660041, Russia; RAS, Krasnoyarsk Sci Ctr SB, Fed Res Ctr, LV Kirenskii Inst Phys, Krasnoyarsk, Russia)
  • Kiselev Evgeniy (Siberian Fed Univ, 79 Svobodnyi Av, Krasnoyarsk 660041, Russia; RAS, Krasnoyarsk Sci Ctr SB, Fed Res Ctr, Inst Biophys, Krasnoyarsk, Russia)
  • Sukovatyi Aleksey (Siberian Fed Univ, 79 Svobodnyi Av, Krasnoyarsk 660041, Russia; RAS, Krasnoyarsk Sci Ctr SB, Fed Res Ctr, Inst Biophys, Krasnoyarsk, Russia)
  • Volova Tatiana (Siberian Fed Univ, 79 Svobodnyi Av, Krasnoyarsk 660041, Russia; RAS, Krasnoyarsk Sci Ctr SB, Fed Res Ctr, Inst Biophys, Krasnoyarsk, Russia)

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