Тип публикации: статья из журнала

Год издания: 2018

Идентификатор DOI: 10.1134/S1607672918020126

Аннотация: A new method for obtaining stable butyrylcholinesterase (BuChE) samples based on the enzyme immobilization in starch and gelatin gels followed by drying is proposed. Coimmobilization of BuChE with the thiol group indicator 5,5'-dithiobis(2-nitrobenzoic) acid did not reduce the activity of BuChE, which allowed us to simplify the proПоказать полностьюcedure and reduce the time of analysis of organophosphorus pesticides. The resulting immobilized samples retained activity for at least 300 days. BuChE samples based on the starch gel showed a greater sensitivity in the determination of pesticides as compared to the samples based on the gelatin gel.

Журнал: DOKLADY BIOCHEMISTRY AND BIOPHYSICS

Выпуск журнала: Vol. 479, Is. 1

Номера страниц: 98-100

ISSN журнала: 16076729

Место издания: NEW YORK

Издатель: MAIK NAUKA/INTERPERIODICA/SPRINGER

• Lonshakova-Mukina V.I. (Siberian Fed Univ, Krasnoyarsk 660041, Russia)
• Esimbekova E.N. (Siberian Fed Univ, Krasnoyarsk 660041, Russia; Russian Acad Sci, Fed Res Ctr, Inst Biophys, Siberian Branch,Krasnoyarsk Res Ctr, Krasnoyarsk 660036, Russia)
• Kratasyuk V.A. (Siberian Fed Univ, Krasnoyarsk 660041, Russia; Russian Acad Sci, Fed Res Ctr, Inst Biophys, Siberian Branch,Krasnoyarsk Res Ctr, Krasnoyarsk 660036, Russia)

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