Тип публикации: статья из журнала
Год издания: 2010
Идентификатор DOI: 10.3390/s101211287
Ключевые слова: bioluminescence, Ca2+-regulated photoprotein, immunoassay, Multiplex assay, PCR-ELISA, Re-engineered photoproteins
Аннотация: Ca2+-regulated photoproteins of luminous marine coelenterates are of interest and a challenge for researchers as a unique bioluminescent system and as a promising analytical instrument for both in vivo and in vitro applications. The proteins are comprehensively studied as to biochemical properties, tertiary structures, bioluminesceПоказать полностьюnce mechanism, etc. This knowledge, along with available recombinant proteins serves the basis for development of unique bioluminescent detection systems that are "self-contained", triggerable, fast, highly sensitive, and non-hazardous. In the paper, we focus on the use of photoproteins as reporters in binding assays based on immunological recognition element-bioluminescent immunoassay and hybridization immunoassay, their advantages and prospects. © 2010 by the authors.
Журнал: Sensors
Выпуск журнала: Т. 10, № 12
Номера страниц: 11287-11300
ISSN журнала: 14248220
Издатель: Molecular Diversity Preservation International