Short-term culture of monocytes as an in vitro evaluation system for bionanomaterials designated for medical use


Тип публикации: статья из журнала

Год издания: 2016

Идентификатор DOI: 10.1016/j.fct.2016.08.025

Ключевые слова: Biocompatible biopolymers, Polyhydroxyalkanoates, Nanomaterials, Nanodiamonds, Fullerenes, Bio-nanomaterials, Monocytes

Аннотация: We studied the feasibility of using a short-term culture of monocytes, isolated from peripheral donor blood, to assess the biological activity of different types of bionanomaterials (BNM): biodegradable polimeric particles, fiber and film substrates of micro- and nano-dimensions, fullerenes (F) and nano diamonds (ND), which are eitПоказать полностьюher currently in use and/or potentially applicable in medicine. Additionally, the effect of creating a protein corona on ND and F particles was investigated. The cellular reduction of (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (WITT) is a well-established tool for assessing the viability/metabolic activity of cells. The scanning electron microscopy assay can detect fine changes in cell morphology. In the present study BNM have been shown to affect; in a size, chemical composition and morphological characteristics-dependent manner, the ability of monocytes to reduce MU as well as their morphology. Moreover, the specific effects of ND and F on MU reduction and cell morphology were exhibited in a dose-dependent manner and sensitive to the formation of surface protein corona. Our results suggest that short-term culture of monocytes is a sensitive model system for assessing the biological effects of BMPs in vitro. (C) 2016 Elsevier Ltd. All rights reserved.

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Выпуск журнала: Vol. 96

Номера страниц: 302-308

ISSN журнала: 02786915

Место издания: OXFORD



  • Shishatskaya E.I. (Siberian Federal University)
  • Menzianova N.G. (Siberian Federal University)
  • Nikitovic D. (Laboratory of Anatomy,Histology,Embryology,School of Medicine,University of Crete)
  • Tzanakakis G.N. (Laboratory of Anatomy,Histology,Embryology,School of Medicine,University of Crete)
  • Vasilievich A.S. (Institute of Physics,Russian Academy of Science,Siberian Division)
  • Tsatsakis A.M. (Laboratory of Toxicology,Medical School,University of Crete)

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