Тип публикации: статья из журнала
Год издания: 2015
Идентификатор DOI: 10.2174/1386207318666150917095903
Ключевые слова: SNP detection, PEXT reaction, photoprotein obelin, bioluminescent microassay, multiplex PCR
Аннотация: Multiplex simultaneous genotyping technique was developed for four polymorphisms in genes coding for blood coagulation factors and homocysteine metabolism which are considered as thrombophilia related mutations: FV Leiden, FII G20210A, MTHFR C677T, and FVII G10976A. It is based on primer extension reaction with the following biolumПоказать полностьюinescent solid-phase microassay. At that, two different in bioluminescence obelin mutants were applied to simultaneous detection of two gene allelic variants. The assay is carried out in microtiter plate format and provides fast and reliable genotyping of four single nucleotide polymorphisms in four different genes within 2.5 hours. A large number of clinical samples were analyzed and the obtained results were found to be in complete correlation with those obtained by using conventional RT-PCR techniques.
Журнал: COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING
Выпуск журнала: Vol. 18, Is. 10
Номера страниц: 930-936
ISSN журнала: 13862073
Место издания: SHARJAH
Издатель: BENTHAM SCIENCE PUBL LTD