Effect of natural polysaccharide matrix-based selenium nanocomposites on phytophthora cactorum and rhizospheric microorganisms


Тип публикации: статья из журнала

Год издания: 2021

Идентификатор DOI: 10.3390/nano11092274

Ключевые слова: acinetobacter guillouiae, antibacterial activity, arabinogalactan, fungicidal effect, nanocomposites, phytophthora cactorum, polysaccharides, potato productivity, pseudomonas oryzihabitans, rhodococcus erythropolis, selenium

Аннотация: We studied the effects of new chemically synthesized selenium (Se) nanocomposites (NCs) based on natural polysaccharide matrices arabinogalactan (AG), starch (ST), and kappa-carrageenan (CAR) on the viability of phytopathogen Phytophthora cactorum, rhizospheric bacteria, and potato productivity in the field experiment. Using transmПоказать полностьюission electron microscopy (TEM), it was shown that the nanocomposites contained nanoparticles varying from 20 to 180 nm in size depending on the type of NC. All three investigated NCs had a fungicidal effect even at the lowest tested concentrations of 50 µg/mL for Se/AG NC (3 µg/mL Se), 30 µg/mL for Se/ST NC (0.5 µg/mL Se), and 39 µg/mL for Se/CAR NC (1.4 µg/mL Se), including concentration of 0.000625% Se (6.25 µg/mL) in the final suspension, which was used to study Se NC effects on bacterial growth of the three common rhizospheric bacteria Acinetobacter guillouiae, Rhodococcus erythropolis and Pseudomonas oryzihabitans isolated from the rhizosphere of plants growing in the Irkutsk Region. The AG-based Se NC (Se/AG NC) and CAR-based Se NC (Se/CAR NC) exhibited the greatest inhibition of fungal growth up to 60% (at 300 µg/mL) and 49% (at 234 µg/mL), respectively. The safe use of Se NCs against phytopathogens requires them to be environmentally friendly without negative effects on rhizospheric microorganisms. The same concentration of 0.000625% Se (6.25 µg/mL) in the final suspension of all three Se NCs (which corresponds to 105.57 µg/mL for Se/AG NC, 428.08 µg/mL for Se/ST NC and 170.30 µg/mL for Se/CAR NC) was used to study their effect on bacterial growth (bactericidal, bacteriostatic, and biofilm formation effects) of the three rhizospheric bacteria. Based on our earlier studies this concentration had an antibacterial effect against the phytopathogenic bacterium Clavibacter sepedonicus that causes diseases of potato ring rot, but did not negatively affect the viability of potato plants at this concentration. In this study, using this concentration no bacteriostatic and bactericidal activity of all three Se NCs were found against Rhodococcus erythropolis based on the optical density of a bacterial suspension, agar diffusion, and intensity of biofilm formation, but Se/CAR and AG NCs inhibited the growth of Pseudomonas oryzihabitans. The cell growth was decrease by 15–30% during the entire observation period, but the stimulation of biofilm formation by this bacterium was observed for Se/CAR NC. Se/AG NC also had bacteriostatic and antibiofilm effects on the rhizospheric bacterium Acinetobacter guillouiae. There was a 2.5-fold decrease in bacterial growth and a 30% decrease in biofilm formation, but Se/CAR NC stimulated the growth of A. guillouiae. According to the results of the preliminary field test, an increase in potato productivity by an average of 30% was revealed after the pre-planting treatment of tubers by spraying them with Se/AG and Se/CAR NCs with the same concentration of Se of 0.000625% (6.25 µg/mL) in a final suspension. The obtained and previously published results on the positive effect of natural matrix-based Se NCs on plants open up prospects for further investigation of their effects on rhizosphere bacteria and resistance of cultivated plants to stress factors. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

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Журнал: Nanomaterials

Выпуск журнала: Vol. 11, Is. 9

Номера страниц: 2274

ISSN журнала: 20794991

Издатель: MDPI


  • Perfileva Alla (Russian Acad Sci, Siberian Inst Plant Physiol & Biochem, Lab Plant Microbe Interact, Siberian Branch, Irkutsk 664033, Russia)
  • Tsivileva Olga M. (Russian Acad Sci, Inst Biochem & Physiol Plants & Microorganisms, Lab Microbiol, Saratov 410049, Russia)
  • Nozhkina Olga A. (Russian Acad Sci, Siberian Inst Plant Physiol & Biochem, Lab Plant Microbe Interact, Siberian Branch, Irkutsk 664033, Russia)
  • Karepova Marina S. (Russian Acad Sci, Siberian Inst Plant Physiol & Biochem, Lab Plant Microbe Interact, Siberian Branch, Irkutsk 664033, Russia)
  • Graskova Irina A. (Russian Acad Sci, Siberian Inst Plant Physiol & Biochem, Lab Plant Microbe Interact, Siberian Branch, Irkutsk 664033, Russia)
  • Ganenko Tatjana (Russian Acad Sci, AE Favorsky Irkutsk Inst Chem, Lab Funct Nanomat, Siberian Branch, Irkutsk 664033, Russia)
  • Sukhov Boris G. (Russian Acad Sci, VV Voevodsky Inst Chem Kinet & Combust, Lab Nanoparticles, Siberian Branch, Novosibirsk 630090, Russia)
  • Krutovsky Konstantin (Georg August Univ Gottingen, Fac Forest Sci & Forest Ecol, Dept Forest Genet & Forest Tree Breeding, Busgenweg 2, D-37077 Gottingen, Germany; Georg August Univ Gottingen, Ctr Integrated Breeding Res CiBreed, Albrecht Thaer Weg 3, D-37075 Gottingen, Germany; Russian Acad Sci, NI Vavilov Inst Gen Genet, Lab Populat Genet, Moscow 119333, Russia; Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Genome Res & Educ Ctr, Lab Forest Genom, Krasnoyarsk 660036, Russia)

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