Тип публикации: статья из журнала
Год издания: 2020
Идентификатор DOI: 10.1134/S1607672920020106
Ключевые слова: bacterial luciferase, bioluminescence, diffusional restriction, enzyme reaction intermediate, molecular dynamics method, stopped-flow technique, viscous microenvironment
Аннотация: Abstract: The effects of medium viscosity on the decay rate of the 4a-hydroperoxyflavin intermediate of the bioluminescent reaction was investigated. It was found that at low concentrations of glycerol or sucrose (viscosity 1.1–1.3 cP) the decay rate rises, whereas a further increase in viscosity to 6.2 cP leads to a decrease in thПоказать полностьюe decay rate following a power function with an exponent of 0.82–0.84. Using molecular dynamics methods, it was shown that the presence of glycerol and sucrose molecules causes a change in the mobility of the amino acid residues in the active center of luciferase, particularly those responsible for binding of flavin. The results obtained are indicative of two opposite effects of viscous media with glycerol and sucrose: (1) destabilization of 4a-hydroperoxyflavin due to a change in the structural and dynamic properties of the protein and (2) stabilization of this intermediate by the decrease in the diffusion rate of its decay products. © 2020, Pleiades Publishing, Ltd.
Журнал: Doklady Biochemistry and Biophysics
Выпуск журнала: Vol. 492, Is. 1
Номера страниц: 162-165
ISSN журнала: 16076729
Издатель: Pleiades Publishing