Тип публикации: статья из журнала (материалы конференций, опубликованные в журналах)
Конференция: International Symposium on Bioluminescence and Chemiluminescence; BOLOGNA, ITALY; BOLOGNA, ITALY
Год издания: 1999
Идентификатор DOI: 10.1002/(SICI)1522-7243(199907/08)14:4<189::AID-BIO527>3.0.CO;2-E
Ключевые слова: enzyme; science education; luciferase; bioluminescence, Bioluminescence, Enzyme, Luciferase, Science education, immobilized enzyme, article, biochemistry, continuing education, education, human, laboratory personnel, luminescence, Education, Continuing, Enzymes, Enzymes, Immobilized, Humans
Аннотация: We describe our experience with laboratory courses in enzymology based on the phenomenon of bioluminescence. The soluble and immobilized enzymes of luminous bacteria are used and the practical enzymological course consists of four main courses: (1) training in measuring the activities of soluble and immobilized enzymes; (2) the invПоказать полностьюestigation of kinetic characteristics (kinetic constants) and enzyme-substrate and enzyme-inhibitor interactions in the bacterial bioluminescent reaction; (3) The testing of physico-chemical characteristics of enzymes (pH, temperature, ion strength, etc.); (4) the effect of inhibitors on enzymes. Training is possible in groups of about ten persons. Our practice work has been introduced in the biological, pedagogical and physical departments of Krasnoyarsk State University. Students of the pedagogical department have created a popular and interesting series of laboratory works for high school children aged 14-17 years. Copyright (C) 1999 John Wiley & Sons, Ltd.
Журнал: LUMINESCENCE
Выпуск журнала: Vol. 14, Is. 4
Номера страниц: 189-192
ISSN журнала: 15227235
Место издания: W SUSSEX
Издатель: JOHN WILEY & SONS LTD