Тип публикации: статья из журнала

Год издания: 2024

Идентификатор DOI: 10.1134/S0006350924700635

Ключевые слова: <b>Keywords:</b> bioluminescent enzymatic bioassay, biochemical analysis, infrared Fourier spectroscopy, saliva, aerobic and anaerobic physical load, sports medicine

Аннотация: Abstract—The saliva of athletes with anaerobic (track and field athletes) and aerobic (swimmers) physical loads was studied. Saliva was collected before (in a state of physical rest) and after training. It was found that physical load significantly increased the concentration of total protein in saliva and the activity of hydrogen ions (pH) in the saliva. Catalase activity in saliva was significantly increased only after aerobic physical load, as opposed to anaerobic load. The bioluminescent saliva index was significantly increased for athletes engaged in aerobic physical load compared to that for an anaerobic load. Saliva biomarkers such as the concentration of lactate and total protein, catalase activity, concentration of calcium, potassium, and magnesium ions, as well as the content of group IV amides, had a significant effect on the enhancement of light emission in a bioluminescent two-enzyme reaction. The content of group IV amides and a complex of oligosaccharides, polysaccharides, phosphatase, and phospholipids in the saliva of athletes engaged in anaerobic physical load could more strongly inhibit bioluminescent glow than the concentration of triene conjugates and the content of group II amides in the saliva of athletes engaged in aerobic physical load. Thus, significant biomarkers of the physiological state of the body have been identified in the saliva of athletes engaged in aerobic or anaerobic physical loads, which were able to affect the integral response of a bioluminescent enzymatic biotest. A change in the integral bioluminescent saliva index, depending on the type of physical load for an athlete, can be used in sports medicine to prevent physical overloads.

Журнал: Biophysics

Выпуск журнала: Т. 69, № 3

Номера страниц: 566-574

ISSN журнала: 00063509

Место издания: Moscow

Издатель: Pleiades Publishing, Inc.

• Malysheva V.V. (Siberian Federal University)
• Stepanova L.V. (Siberian Federal University)
• Vyshedko A.M. (Siberian Federal University)
• Bel'skaya L.V. (Omsk State Pedagogical University)
• Sarf E.A. (Omsk State Pedagogical University)
• Khalzhanova Z. (Siberian Federal University)
• Kolenchukova O.A. (Krasnoyarsk State Agrarian University)
• Kratasyuk V.A. (Institute of Biophysics, Krasnoyarsk Science Center Federal Research Center, Siberian Branch, Russian Academy of Sciences)

• Ядро РИНЦ (eLIBRARY.RU)