Тип публикации: статья из журнала
Год издания: 2024
Идентификатор DOI: 10.1134/S0006350924700465
Ключевые слова: bacterial bioluminescence, bioluminescence quantum yield, luciferase, media effect
Аннотация: Based on the data on the transient kinetics of the bioluminescent reaction catalyzed by <i>P. leiognathi</i> luciferase in media with polyols and sugars, the relative quantum yield of bioluminescence in this reaction per substrate molecule was determined using mathematical modeling. It was obtained that in some media the relative qПоказать полностьюuantum yield per aldehyde molecule increases compared to the value in the buffer: by 18 and 33% in the presence of glycerol and sucrose, respectively. Molecular dynamics methods were used to analyze the side-chain conformation of αHis44 – a residue of bacterial luciferases that is known to play an essential role in catalysis. It was found that in the presence of all cosolvents an increased frequency of occurrence of the αHis44 conformation, which is optimal for catalysis, is observed. This effect can contribute to the observed increase in the quantum yield of bioluminescence of the studied reaction in viscous media.
Журнал: Biophysics
Выпуск журнала: Т. 69, № 3
Номера страниц: 380-389
ISSN журнала: 00063509
Место издания: Moscow
Издатель: Pleiades Publishing, Inc.