Тип публикации: статья из журнала

Год издания: 2020

Идентификатор DOI: 10.1002/cmdc.202000050

Аннотация: The Front Cover shows the interaction epitopes of the extracellular structure of human C-Met receptor protein complexes with two DNA-aptamers of 60 and 64 bases, produced in vitro by the SELEX (Systematic Evolution of Ligands by Exponential Enrichment) procedure. The C-Met protein has been recently recognized as a highly useful bioПоказать полностьюmarker in cancer diagnosis. Using proteolytic affinity–mass spectrometry in combination with SPR biosensor analysis, epitope identifications with high affinities were obtained for DNA aptamers with a single linear peptide (aptamer CLN0004; upper right), and a discontinuous assembled epitope (aptamer CLN0003; upper left). The molecular epitopes identified by mass spectrometry for the first time of DNA aptamers as potential antibody alternatives, render them of high interest for cellular diagnostics. More information can be found in the Full Paper by Loredana Lupu, Michael Przybylski et al. on page 363 in Issue 4, 2020 (DOI: 10.1002/cmdc.201900489).

Журнал: ChemMedChem: Chemistry Enabling Drug Discovery

Выпуск журнала: Т. 15, № 4

Номера страниц: 338-338

ISSN журнала: 18607179

Издатель: VCH Verlag GmbH & CO. KGaA

• Lupu Loredana (Steinbeis Centre for Biopolymer Analysis and Biomedical Mass Spectrometry Marktstraße 29 65428 Rüsselsheim am Main Germany)
• Wiegand Pascal (Steinbeis Centre for Biopolymer Analysis and Biomedical Mass Spectrometry Marktstraße 29 65428 Rüsselsheim am Main Germany)
• Hüttmann Nico (Department of Chemistry and Biomolecular Sciences University of Ottawa Ottawa, ON K1N 6N5 Canada)
• Rawer Stephan (Steinbeis Centre for Biopolymer Analysis and Biomedical Mass Spectrometry Marktstraße 29 65428 Rüsselsheim am Main Germany)
• Kleinekofort Wolfgang (Dept. of Engineering Sciences Rhein Main University 65428 Rüsselsheim am Main Germany)
• Shugureva Irina (Federal Research Center “Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Science” Laboratory for Digital Controlled Drugs and Theranostics Krasnoyarsk 660036 Russia)
• Kichkailo Anna S. (Federal Research Center “Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Science” Laboratory for Digital Controlled Drugs and Theranostics Krasnoyarsk 660036 Russia)
• Tomilin Felix N. (Kirensky Institute of Physics Russian Academy of Sciences Siberian Branch Krasnoyarsk 660036 Russia)
• Lazarev Alexander (Pressure Biosciences Inc. 14 Norfolk Ave. South Easton, MA 02375 USA)
• Berezovski Maxim V. (Department of Chemistry and Biomolecular Sciences University of Ottawa Ottawa, ON K1N 6N5 Canada)
• Przybylski Michael (Steinbeis Centre for Biopolymer Analysis and Biomedical Mass Spectrometry Marktstraße 29 65428 Rüsselsheim am Main Germany)

• Ядро РИНЦ (eLIBRARY.RU)