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Silica-Coated Iron Oxide Nanoparticles for DNA Isolation for Molecular Genetic Studies in Hematology : научное издание

Описание

Тип публикации: статья из журнала

Год издания: 2021

Идентификатор DOI: 10.1089/gtmb.2021.0067

Ключевые слова: magnetic nanoparticles, dna isolation, myeloproliferative neoplasms, jak2 v617f

Аннотация: Aim: To develop magnetic nanoparticles (MNPs) based on iron oxide for DNA isolation from blood cells for quantitative molecular genetic analyses of the V617F mutation in the Januskinase 2 (JAK2) gene. Materials and Methods: MNPs were synthesized by the coprecipitation method and coated with tetraethyl orthosilicate (TEOS). The sizeПоказать полностьюand shape of the complexes were estimated using transmission electron microscopy. Twenty blood samples from patients with myeloproliferative disorders were used for DNA isolation with the MNPs. DNA quality and compatibility for molecular genetic studies of the JAK2 V617F mutation were investigated by gel electrophoresis and real-time polymerase chain reaction (RT-PCR). Results: The average amount of DNA isolated from 150 mu L of whole blood was 75.2 ng when MNPs were used and 72.5 ng when standard silica sorbent was used. There was no DNA damage observed after interaction with MNPs. RT-PCR demonstrated similar values for the JAK2 V617F mutant DNA ratios in the samples after DNA isolation with MNPs and by standard sorption on silica. Conclusions: MNPs with silicate capsules of sufficient thickness were obtained and the undesirable damaging effect of iron oxides on nucleic acids during isolation from cells were eliminated. Designed MNPs allow obtaining intact DNA for molecular genetic studies using the example of the JAK2 V617F for study.

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Издание

Журнал: GENETIC TESTING AND MOLECULAR BIOMARKERS

Выпуск журнала: Vol. 25, Is. 9

Номера страниц: 611-614

ISSN журнала: 19450265

Место издания: NEW ROCHELLE

Издатель: MARY ANN LIEBERT, INC

Персоны

  • Tyumentseva Anna (RAS, Krasnoyarsk Sci Ctr, Fed Res Ctr, Siberian Branch, Akademgorodok 50,Str 12-2, Krasnoyarsk 660036, Russia)
  • Yaroslavtsev Roman N. (RAS, Krasnoyarsk Sci Ctr, Fed Res Ctr, Siberian Branch, Akademgorodok 50,Str 12-2, Krasnoyarsk 660036, Russia; Fed Res Ctr KSC SB RAS, Kirensky Inst Phys, Krasnoyarsk, Russia)
  • Stolyar Sergey (RAS, Krasnoyarsk Sci Ctr, Fed Res Ctr, Siberian Branch, Akademgorodok 50,Str 12-2, Krasnoyarsk 660036, Russia; Fed Res Ctr KSC SB RAS, Kirensky Inst Phys, Krasnoyarsk, Russia; Siberian Fed Univ, Krasnoyarsk, Russia)
  • Saitova Alina T. (Fed Res Ctr KSC SB RAS, Kirensky Inst Phys, Krasnoyarsk, Russia)
  • Tyutrina Ekaterina S. (RAS, Krasnoyarsk Sci Ctr, Fed Res Ctr, Siberian Branch, Akademgorodok 50,Str 12-2, Krasnoyarsk 660036, Russia; Fed Med Biol Agcy, Fed Siberian Res Clin Ctr, Fed State Financed Inst, Krasnoyarsk, Russia)
  • Gorbenko Alexey S. (RAS, Krasnoyarsk Sci Ctr, Fed Res Ctr, Siberian Branch, Akademgorodok 50,Str 12-2, Krasnoyarsk 660036, Russia; Natl Res Ctr Hematol, Dept Hlth, Krasnoyarsk Branch, Krasnoyarsk, Russia)
  • Stolyar Marina A. (RAS, Krasnoyarsk Sci Ctr, Fed Res Ctr, Siberian Branch, Akademgorodok 50,Str 12-2, Krasnoyarsk 660036, Russia; Natl Res Ctr Hematol, Dept Hlth, Krasnoyarsk Branch, Krasnoyarsk, Russia)
  • Olkhovskiy Igor A. (RAS, Krasnoyarsk Sci Ctr, Fed Res Ctr, Siberian Branch, Akademgorodok 50,Str 12-2, Krasnoyarsk 660036, Russia; Natl Res Ctr Hematol, Dept Hlth, Krasnoyarsk Branch, Krasnoyarsk, Russia)

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