Тип публикации: статья из журнала
Год издания: 2024
Идентификатор DOI: 10.1134/S1021443724606700
Ключевые слова: <i>Larix sibirica</i>, clone tree, cell lines, microsatellite markers, somatic embryogenesis
Аннотация: The paper deals with the investigation of genetic identity of genotypes by 21 microsatellite loci of a donor tree, embryogenic cell line (CL6) produced from a donor tree, and clones grown from CL6 and embryogenic CL22.27.1 for which the explant (zygotic embryo) was produced as a result of controlled pollination of a clone with pollПоказать полностьюen from a maternal donor tree. Genotyping by nuclear microsatellite loci of a donor tree and CL6 showed a partial identity of detected alleles by the majority of examined loci. Variability of individual loci in the samples indicates manifestation of paternal genotype caused by introduced pollen inevitable for free pollination. Genotypes of clone needles entirely corresponded to CL6. In CL22.27.1, most loci contained alleles lacking in parental genotypes. Only two loci were identical to parental genotypes. The high frequency of mutations in the produced cell line indicates its genomic instability.
Журнал: Russian Journal of Plant Physiology
Выпуск журнала: Т. 71, № 6
Номера страниц: 1-7
ISSN журнала: 10214437
Место издания: Москва
Издатель: Плеадес Паблишинг, Лтд