Тип публикации: статья из журнала

Год издания: 2024

Идентификатор DOI: 10.1016/j.giant.2024.100314

Аннотация: In this work, for the first time, films were obtained based on biodegradable polyhydroxyalkanoates (PHAs) - a copolymer of 3-hydroxybutyrate-co-3-hydroxyvalerate P(3HB-co-3HV) and a copolymer of 3-hydroxybutyrate-co-4-hydroxybutyrate P(3HB-co-4HB), containing drugs of different chemical structure and action - antibiotics (ceftazidiПоказать полностьюme, doripenem), antiseptic (chlorhexidine) and tissue regeneration stimulator (Actovegin) for drug delivery and cell culture. Using SEM, AFM and measuring the contact angles of water wetting, it is shown how PHAs composition and loading of films of P(3HB-co-3HV) and P(3HB-co-4HB) with drugs affect the porosity, roughness indicators and adhesive properties of the surface. The constructed films are a depot form of drug, the release of which in vitro is realized for a long time, without burst releases, corresponds to Korsmeyer-Peppas and Higuchi models with diffusion character. Films loaded with antibiotics have pronounced antibacterial activity and suppress the development of pathogens S. aureus and E. coli, without a pronounced negative effect on the adhesion and proliferation of epidermal cells. In the cultures of human keratinocytes HaCaT it was shown that the presence of actovegin, as well as ceftazidime in the films, exhibits a stimulating effect, increasing their number. The results demonstrate the suitability of the depot-films for cellular technologies and are promising for the reconstruction of tissues complicated by infection.

Журнал: Giant

Выпуск журнала: Т. 19

Номера страниц: 100314

ISSN журнала: 26665425

Издатель: Elsevier B.V.

• Murueva Anastasiya V. (Siberian Federal University, 79 Svobodnyi av., Krasnoyarsk 660041, Russia)
• Dudaev Alexey E. (Siberian Federal University, 79 Svobodnyi av., Krasnoyarsk 660041, Russia)
• Shishatskaya Ekaterina I. (Infochemistry Scientific Centre, ITMO University, Lomonosova St. 9, St. Petersburg 191002, Russia)
• Ghorabe Fares D.E. (Infochemistry Scientific Centre, ITMO University, Lomonosova St. 9, St. Petersburg 191002, Russia)
• Nemtsev Ivan V. (L.V. Kirensky Institute of Physics, Federal Research Center "Krasnoyarsk Science Center SB RAS, 50/12 Akademgorodok, Krasnoyarsk, 660036, Russia)
• Lukyanenko Anna V. (L.V. Kirensky Institute of Physics, Federal Research Center "Krasnoyarsk Science Center SB RAS, 50/12 Akademgorodok, Krasnoyarsk, 660036, Russia)
• Volova Tatiana G. (Siberian Federal University, 79 Svobodnyi av., Krasnoyarsk 660041, Russia)

• Ядро РИНЦ (eLIBRARY.RU)