Тип публикации: статья из журнала

Год издания: 2024

Идентификатор DOI: 10.1021/jacs.4c06716

Аннотация: Gliomas remain challenging brain tumors to treat due to their infiltrative nature. Accurately identifying tumor boundaries during surgery is crucial for successful resection. This study introduces an innovative intraoperative visualization method utilizing surgical fluorescence microscopy to precisely locate tumor cell disseminatioПоказать полностьюn. Here, the focus is on the development of a novel contrasting agent (IR-Glint) for intraoperative visualization of human glial tumors comprising infrared-labeled Glint aptamers. The specificity of IR-Glint is assessed using flow cytometry and microscopy on primary cell cultures. In vivo effectiveness is studied on mouse and rabbit models, employing orthotopic xenotransplantation of human brain gliomas with various imaging techniques, including PET/CT, in vivo fluorescence visualization, confocal laser scanning, and surgical microscopy. The experiments validate the potential of IR-Glint for the intraoperative visualization of gliomas using infrared imaging. IR-Glint penetrates the blood-brain barrier and can be used for both intravenous and surface applications, allowing clear visualization of the tumor. The surface application directly to the brain reduces the dosage required and mitigates potential toxic effects on the patient. The research shows the potential of infrared dye-labeled aptamers for accurately visualizing glial tumors during brain surgery. This novel aptamer-assisted fluorescence-guided surgery (AptaFGS) may pave the way for future advancements in the field of neurosurgery.

Журнал: Journal of the American Chemical Society

Выпуск журнала: Т. 146, № 36

Номера страниц: 24989-25004

ISSN журнала: 00027863

Издатель: American Chemical Society

• Zamay Galina (Federal Research Center “Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences”, Krasnoyarsk 660036, Russia)
• Koshmanova Anastasia (Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk 660022, Russia)
• Narodov Andrey (Aptamerlab LLC, Krasnoyarsk 660042, Russia)
• Gorbushin Anton (Krasnoyarsk Inter-District Ambulance Hospital Named after N.S. Karpovich, 17 Kurchatova, Krasnoyarsk 660062, Russia)
• Voronkovskii Ivan (Krasnoyarsk Inter-District Ambulance Hospital Named after N.S. Karpovich, 17 Kurchatova, Krasnoyarsk 660062, Russia)
• Grek Daniil (Aptamerlab LLC, Krasnoyarsk 660042, Russia)
• Luzan Natalia (Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk 660022, Russia)
• Kolovskaya Olga (Federal Research Center “Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences”, Krasnoyarsk 660036, Russia)
• Shchugoreva Irina (Federal Research Center “Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences”, Krasnoyarsk 660036, Russia)
• Artyushenko Polina (Federal Research Center “Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences”, Krasnoyarsk 660036, Russia)
• Glazyrin Yury (Federal Research Center “Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences”, Krasnoyarsk 660036, Russia)
• Fedotovskaya Victoriya (Federal Research Center “Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences”, Krasnoyarsk 660036, Russia)
• Kuziakova Olga (School of Medicine and Life Sciences, Far Eastern Federal University, Vladivostok 690922, Russia)
• Veprintsev Dmitry (Federal Research Center “Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences”, Krasnoyarsk 660036, Russia)
• Belugin Kirill (Federal Siberian Research Clinical Centre under the Federal Medical Biological Agency, Krasnoyarsk 660130, Russia)
• Lukyanenko Kirill (Federal Research Center “Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences”, Krasnoyarsk 660036, Russia)
• Nikolaeva Elena (Federal Research Center “Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences”, Krasnoyarsk 660036, Russia)
• Kirichenko Andrey (Aptamerlab LLC, Krasnoyarsk 660042, Russia)
• Lapin Ivan (Laboratory of Advanced Materials and Technology, Tomsk State University, Tomsk 634050, Russia)
• Khorzhevskii Vladimir (Krasnoyarsk Regional Pathology-Anatomic Bureau, Partizana Zheleznyaka, Krasnoyarsk 660022, Russia)
• Semichev Evgeniy (Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk 660022, Russia)
• Mohov Alexey (Federal Research Center “Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences”, Krasnoyarsk 660036, Russia)
• Kirichenko Daria (Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk 660022, Russia)
• Tokarev Nikolay (Federal Siberian Research Clinical Centre under the Federal Medical Biological Agency, Krasnoyarsk 660130, Russia)
• Chanchikova Natalia (Federal Siberian Research Clinical Centre under the Federal Medical Biological Agency, Krasnoyarsk 660130, Russia)
• Krat Alexey (Krasnoyarsk Regional Clinical Cancer Center, 16 1-ya Smolenskaya, Krasnoyarsk 660133, Russia)
• Zukov Ruslan (Krasnoyarsk Regional Clinical Cancer Center, 16 1-ya Smolenskaya, Krasnoyarsk 660133, Russia)
• Bakhtina Varvara (Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk 660022, Russia)
• Shnyakin Pavel (Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk 660022, Russia)
• Shesternya Pavel (Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk 660022, Russia)
• Tomilin Felix (Kirensky Institute of Physics, Krasnoyarsk 660036, Russia)
• Kosinova Aleksandra (Federal Research Center “Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences”, Krasnoyarsk 660036, Russia)
• Svetlichnyi Valery (Laboratory of Advanced Materials and Technology, Tomsk State University, Tomsk 634050, Russia)
• Zamay Tatiana (Federal Research Center “Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences”, Krasnoyarsk 660036, Russia)
• Kumeiko Vadim (National Scientific Center of Marine Biology Far Eastern Branch of Russian Academy of Sciences)
• Mezko Vasily (Aptamerlab LLC, Krasnoyarsk 660042, Russia)
• Berezovski Maxim V. (Department of Chemistry and Biomolecular Sciences, University of Ottawa, Ottawa, Ontario K1N 6N5, Canada)
• Kichkailo Anna (Federal Research Center “Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences”, Krasnoyarsk 660036, Russia)

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