Тип публикации: статья из журнала
Год издания: 2015
Идентификатор DOI: 10.1016/j.snb.2015.02.061
Ключевые слова: Bioluminescence, Body fluids, Carrier concentration, Enzymes, Starch, Bioluminescent biosensor, Bovine serum albumins, Dithiothreitol, Maximum permissible concentration, Mercaptoethanol, Oxidoreductases, Storage stability, Toxic substances, Biosensors
Аннотация: The biomodule of bioluminescent biosensor based on a coupled enzyme system NADH:FMN-oxidoreductase and luciferase, co-immobilized with substrates in dried starch or gelatin gels, has been developed. We studied the impact of several stabilizers-dithiothreitol (DTT), bovine serum albumin (BSA) and mercaptoethanol (ME) on the biomodulПоказать полностьюe's activity, storage stability and sensitivity to toxic substances. The inclusion of stabilizers increases the activity of the biological module by more than 150%. To achieve the combination of high activity, prolonged storage time and acute sensitivity to toxic substances within maximum permissible concentration we used starch gel as a carrier adding 100 mu M DTT to the immobilized preparation. The gelatin-based biological module had greater storage stability than the starch-based one but demonstrated less sensitivity to toxic substances. (C) 2015 Elsevier B.V. All rights reserved.
Журнал: SENSORS AND ACTUATORS B-CHEMICAL
Выпуск журнала: Vol. 213
Номера страниц: 244-247
ISSN журнала: 09254005
Место издания: LAUSANNE
Издатель: ELSEVIER SCIENCE SA