Тип публикации: статья из журнала
Год издания: 2020
Идентификатор DOI: 10.14258/turczaninowia.23.3.11
Ключевые слова: adventitious shoot regeneration, axillary shoot proliferation, rhododendron sichotense, thidiazuron
Аннотация: Rhododendron sichotense Pojark. (Ericaceae) is a winter-hardy species native to the Russian Far East, highly valued as an attractive ornamental woody plant. In order to establish of in vitro culture without the problem with exudation of polyphenols and to induce multiplication from leave and shoot explants of field grown plants, thПоказать полностьюe procedure including the pre-cultivation of explants on hormone free Anderson Medium (AMO) for 3 days followed by cultivation on AM supplemented with varying thidiazuron (TDZ) concentrations (0.1, 0.5, 1.0 mu M) was developed. The elongation on hormone-free AMO allowed obtaining the healthy shoots without any anomalies. The best response in terms of the regeneration frequency (50 %), the shoot number per explant (41.60 +/- 5.25) and the shoot length (12.20 +/- 1.04 mm) was achieved under 0.5 mu M of TDZ from leaf explants. Rhododendron sichotense Pоjаrk. (Ericaceae) is a winter-hardy species native to the Russian Far East, highly valued as an attractive ornamental woody plant. In order to establish of in vitro culture without the problem with exudation of polyphenols and to induce multiplication from leave and shoot explants of field grown plants, the procedure including the pre-cultivation of explants on hormone free Andersоn Medium (AM0) for 3 days followed by cultivation on AM supplemented with varying thidiazuron (TDZ) concentrations (0.1, 0.5, 1.0 µM) was developed. The elongation on hormone-free AM0 allowed obtaining the healthy shoots without any anomalies. The best response in terms of the regeneration frequency (50 %), the shoot number per explant (41.60 ± 5.25) and the shoot length (12.20 ± 1.04 mm) was achieved under 0.5 µM of TDZ from leaf explants. © 2020 Altai State University. All rights reserved.
Журнал: TURCZANINOWIA
Выпуск журнала: Vol. 23, Is. 3
Номера страниц: 106-111
ISSN журнала: 15607259
Место издания: Barnaul
Издатель: Altai State Univ