Тип публикации: статья из журнала

Год издания: 2022

Идентификатор DOI: 10.1080/22221751.2021.2011625

Аннотация: In order to assess the risk of SARS-CoV-2 infection, transmission and reservoir development in swine, we combined results of an experimental and two observational studies. First, intranasal and intratracheal challenge of eight pigs did not result in infection, based on clinical signs and PCR on swab and lung tissue samples. Two serПоказать полностьюum samples returned a low positive result in virus neutralization, in line with findings in other infection experiments in pigs. Next, a retrospective observational study was performed in the Netherlands in the spring of 2020. Serum samples (N =417) obtained at slaughter from 17 farms located in a region with a high human case incidence in the first wave of the pandemic. Samples were tested with protein micro array, plaque reduction neutralization test and receptor-binding-domain ELISA. None of the serum samples was positive in all three assays, although six samples from one farm returned a low positive result in PRNT (titers 40-80). Therefore we conclude that serological evidence for large scale transmission was not observed. Finally, an outbreak of respiratory disease in pigs on one farm, coinciding with recent exposure to SARS-CoV-2 infected animal caretakers, was investigated. Tonsil swabs and paired serum samples were tested. No evidence for infection with SARS-CoV-2 was found. In conclusion, Although in both the experimental and the observational study few samples returned low antibody titer results in PRNT infection with SARS-CoV-2 was not confirmed. It was concluded that sporadic infections in the field cannot be excluded, but large-scale SARS-CoV-2 transmission among pigs is unlikely.

Журнал: Emerging Microbes and Infections

Выпуск журнала: Т. 11, № 1

Номера страниц: 91-94

ISSN журнала: 22221751

• Sikkema Reina S. (Department Viroscience, Erasmus Medical Centre, Rotterdam, The Netherlands)
• Tobias Tijs (Department Population Health Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands)
• Oreshkova Nadia (Wageningen Bioveterinary Research, Lelystad, The Netherlands)
• de Bruin Erwin (Department Viroscience, Erasmus Medical Centre, Rotterdam, The Netherlands)
• Okba Nisreen (Department Viroscience, Erasmus Medical Centre, Rotterdam, The Netherlands)
• Chandler Felicity (Department Viroscience, Erasmus Medical Centre, Rotterdam, The Netherlands)
• Hulst Marcel M. (Wageningen Bioveterinary Research, Lelystad, The Netherlands)
• Rodon Jordi (IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Bellaterra (Cerdanyola del Vallès), Spain)
• Houben Manon (Royal GD Animal Health, Deventer, The Netherlands)
• van Maanen Kees (Royal GD Animal Health, Deventer, The Netherlands)
• Bultman Hans (Royal GD Animal Health, Deventer, The Netherlands)
• Meester Marina (Department Population Health Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands)
• Gerhards Nora M. (Wageningen Bioveterinary Research, Lelystad, The Netherlands)
• Bouwknegt Martijn (Vion Food Group, Boxtel, The Netherlands)
• Urlings Bert (Vion Food Group, Boxtel, The Netherlands)
• Haagmans Bart (Department Viroscience, Erasmus Medical Centre, Rotterdam, The Netherlands)
• Kluytmans Jan (Julius Center for Health Sciences and Primary Care, University Medical Center Utrecht, Utrecht University, Utrecht, The Netherlands)
• GeurtsvanKessel Corine H. (Department Viroscience, Erasmus Medical Centre, Rotterdam, The Netherlands)
• van der Poel Wim H.M. (Wageningen Bioveterinary Research, Lelystad, The Netherlands)
• Koopmans Marion P.G. (Department Viroscience, Erasmus Medical Centre, Rotterdam, The Netherlands)
• Stegeman Arjan (Department Population Health Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands)

• Ядро РИНЦ (eLIBRARY.RU)