Тип публикации: доклад, тезисы доклада, статья из сборника материалов конференций
Конференция: Saratov Fall Meeting; Saratov, RUSSIA; Saratov, RUSSIA
Год издания: 2004
Идентификатор DOI: 10.1117/12.578359
Ключевые слова: carboxyhemoglobin; carbon monoxide; efficiency of photodissociation; laser photolysis
Аннотация: Assessment of the carboxyhemoglobin photodissociation has been performed under the native conditions. This investigation has a great importance for the development and creation of completely new approach for the treatment of carbon monoxide poisoning based on the photoinduced dissociation of carboxyhemoglobin. Photodissociation wasПоказать полностьюregistered on the experimental setup with crossing laser beams where pulsed Nd:YAG laser at the second harmonics wavelength (lambda=532 nm) was used as a source of photolyzing radiation. Buffered solutions of whole human peripheral blood (PBS, pH=7.4) and diluted hemolized human peripheral blood were used. We found optimal parameters for the registration of the photodissociation such as using of buffered solutions of the whole human peripheral blood with the concentration of carboxyhemoglobin around 50%, detection of dissociation of carboxyhemoglobin at the maximum of absorption within the Soret's band (435 nm). Dependence of photodissociation efficiency on the concentration of the complex in the tested solutions, as well as on the photolysis radiation intensity in both types of solutions was proved.
Журнал: SARATOV FALL MEETING 2003: OPTICAL TECHNOLOGIES IN BIOPHYSICS AND MEDICINE V
Выпуск журнала: Vol. 5474
Номера страниц: 88-
ISSN журнала: 0277786X
Место издания: BELLINGHAM
Издатель: SPIE-INT SOC OPTICAL ENGINEERING