Synthesis of silver nanoparticles by endosymbiont Pseudomonas fluorescens CA 417 and their bactericidal activity


Тип публикации: статья из журнала

Год издания: 2016

Идентификатор DOI: 10.1016/j.enzmictec.2016.10.004

Ключевые слова: Endosymbiont, Silver nanoparticles, Pseudomonas fluorescens CA 417, Coffea arabica L, Antibacterial activity, Kanamycin, Absorption spectroscopy, Antimicrobial agents, Bacteria, Bactericides, Dynamic light scattering, Energy dispersive spectroscopy, Fourier transform infrared spectroscopy, Light scattering, Metal nanoparticles, Microorganisms, Nanoparticles, Particle size, Pathogens, Spectrum analysis, Synthesis (chemical), X ray diffraction analysis, X ray spectroscopy, Anti-bacterial activity, Kanamycins, Pseudomonas fluorescens, Silver

Аннотация: The present study emphasizes on biogenic synthesis of silver nanoparticles and their bactericidal activity against human and phytopathogens. Nanoparticle synthesis was performed using endosymbiont Pseudomonas fluorescens CA 417 inhabiting Coffea arabica L Synthesized nanoparticles were characterized using hyphenated spectroscopic tПоказать полностьюechniques such as UV-vis spectroscopy which revealed maximum absorption 425 nm. Fourier transform infrared spectroscopy (FTIR) analysis revealed the possible functional groups mediating and stabilizing silver nanoparticles with predominant peaks occurring at 3346 corresponding to hydroxyl group, 1635 corresponding carbonyl group and 680 to aromatic group. X-ray diffraction (XRD) analysis revealed the Bragg's diffraction pattern with distinct peaks at 38 degrees 44 degrees, 64 degrees and 78 degrees revealing the face-centered cubic (fcc) metallic crystal corresponding to the (111), (200), (220) and (311) facets of the crystal planes at 2 theta angle. The energy dispersive X-ray spectroscopy (EDS) analysis revealed presence of high intense absorption peak at 3 keV is a typical characteristic of nano-crystalline silver which confirmed the presence of elemental silver. TEM analysis revealed the size of the nanoparticles to be in the range 5-50 nm with polydisperse nature of synthesized nanoparticles bearing myriad shapes. The particle size determined by Dynamic light scattering (DLS) method revealed average size to be 20.66 nm. The synthesized silver nanoparticles exhibited significant antibacterial activity against panel of test pathogens. The results showed Klebsiella pneumoniae (MTCC 7407) and Xanthomonas carnpestris to be more sensitive among the test human pathogen and phyto-pathogen respectively. The study also reports synergistic effect of silver nanoparticles in combination with kanamycin which displayed increased fold activity up to 58.3% against Klebsiella pneumoniae (MTCC 7407). The results of the present investigation are promising enough and attribute towards growing scientific knowledge on development of new antimicrobial agents to combat drug resistant microorganisms. The study provides insight on emerging role of endophytes towards reduction of metal salts to synthesize nanoparticles. (C) 2016 Elsevier Inc, All rights reserved.

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Выпуск журнала: Vol. 95

Номера страниц: 128-136

ISSN журнала: 01410229

Место издания: NEW YORK



  • Syed Baker (Univ Mysore, Dept Studies Microbiol, Bionanotechnol Lab, Mysore, Karnataka, India; Siberian Fed Univ, Lab Biotechnol New Mat, Siberia, Russia)
  • Prasad Nagendra M. N. (JSS Sci & Technol Univ, Dept Biotechnol, JSS Tech Inst Campus, Mysore 570006, Karnataka, India)
  • Dhananjaya B.L. (Jain Univ, CET, Toxicol & Drug Discovery Unit, Ramanagara 562112, India)
  • Mohan Kumar K. (Univ Nacl Autonoma Mexico, Inst Ciencias Fis, Ave Univ S-N, Cuernavaca 62210, Morelos, Mexico; Madanapalle Inst Technol & Sci, Dept Chem, POB 14,Kadiri Rd,Angallu 5, Madanapalle 517325, Andhra Pradesh, India)
  • Yallappa S. (BMS Coll Engn, BMS R&D Ctr, Bangalore 560019, Karnataka, India)
  • Satish S. (Univ Mysore, Dept Studies Microbiol, Bionanotechnol Lab, Mysore, Karnataka, India)