Тип публикации: статья из журнала
Год издания: 2024
Идентификатор DOI: 10.1134/S2635167624600731
Аннотация: Aptamers, short oligonucleotides, are capable of high-affinity binding to targets due to their unique structure. Shortening the aptamer while maintaining the active site will increase the affinity and reduce the cost of synthesis. Using the example of the aptamer LC-224, a method for rational optimization of its length and verificaПоказать полностьюtion of the validity of the developed approach is tested. The use of computer modeling and small-angle X‑ray scattering shows the possibility of optimizing the aptamer structure by removing nucleotides that do not participate in binding to the target. It is shown that truncation of the aptamer does not reduce the affinity and specificity of the DNA aptamer. Thus, theoretical and experimental studies demonstrate successful experience in optimizing the structure of a DNA aptamer by shortening it without compromising its affinity and specificity for its target.
Журнал: Nanobiotechnology Reports
Выпуск журнала: Т. 19, № 2
Номера страниц: 183-187
ISSN журнала: 26351676
Место издания: Road Town
Издатель: Pleiades Publishing, Ltd.