Biosynthesis of multi-component polyhydroxyalkanoates by the bacterium Wautersia eutropha

Описание

Тип публикации: доклад, тезисы доклада, статья из сборника материалов конференций

Конференция: European Symposium on Biopolymers; Kusadasi, TURKEY; Kusadasi, TURKEY

Год издания: 2008

Идентификатор DOI: 10.1002/masy.200850901

Ключевые слова: autotrophic and mixotrophic growth, multi-component polyhydroxyalkanoates, Wautersia eutropha, Bacteriology, Biochemical engineering, Biochemistry, Biopolymers, Biotechnology, Carboxylic acids, Fatty acids, Ketones, Monomers, Polymers, Carbon chains, Carbon nutritions, Even numbers, Growth conditions, Hydroxybutyrate, Hydroxyvalerate, Mixotrophic, Odd numbers, Physico-chemical properties, Polyhydroxyalkanoates, Synthesis of, Wild types, Acids

Аннотация: The study addresses the effect of different conditions of carbon nutrition on synthesis of polyhydroxyalkanoates by the bacterium Wautersia eutropha. In experiments with two wild type strains (H16 and 85786), it has been first found that under mixotrophic growth conditions -CO2+ co-substrate (alkanoic acids) - bacteria can synthesiПоказать полностьюze multi-component PHAs, consisting of short- and medium-chain-length monomers with carbon chains containing 4 to 8 atoms. It has been shown that PHA composition is determined by the type of the co-substrate. Fatty acids with odd number of carbons induce bacteria to synthesize four- and five-component PHAs with hydroxybutyrate, hydroxyvalerate as major monomers and hydroxyhexanoate, hydroxyheptanoate and hydroxyoctanoate as minor, occasionally occurring, ones. Fatty acids with even number of carbons induce synthesis of not only their respective monomers (hydroxyhexanoate and hydroxyoctanoate) but also hydroxyvalerate, making possible synthesis of four-component PHAs, containing hydroxybutyrate and hydroxyhexanoate as major components (up to 18 mol%). A family of short- and medium-chain-length four- and five-component PHAs were synthesized and their physicochemical properties examined.

Ссылки на полный текст

Издание

Журнал: MACROMOLECULAR SYMPOSIA

Выпуск журнала: Vol. 269

Номера страниц: 1-7

ISSN журнала: 10221360

Место издания: WEINHEIM

Издатель: WILEY-V C H VERLAG GMBH

Авторы

  • Volova T.G. (Siberian Federal University)
  • Kalacheva G.S. (Institute of Biophysics,SB,RAS)
  • Steinbuchel A. (Institute of Molecular Microbiology and Biotechnology)

Вхождение в базы данных