Coelenterazine-Dependent Luciferases: Properties and Application in Molecular Analysis

Описание

Тип публикации: статья из журнала

Год издания: 2024

Идентификатор DOI: 10.3103/S0027131424700184

Ключевые слова: bioluminescence, coelenterazine, luciferase, Ca<sup>2+</sup>-regulated photoprotein, analytical systems

Аннотация: This review presents the data on the structure of bioluminescent systems of marine animals that use coelenterazine-dependent luciferases as light-emitting proteins. It is shown that these luciferases and their genetic variants with new useful properties are successfully used as reporter molecules in a variety of analytical systems Показать полностьюin vitro and in vivo due to the availability, stability, and high quantum yield of the reactions. Their application ensures a high level of sensitivity, simple design, and fast analysis.

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Издание

Журнал: Moscow University Chemistry Bulletin

Выпуск журнала: Т. 79, 3

Номера страниц: 203-210

ISSN журнала: 00271314

Место издания: Moscow

Издатель: Allerton Press, Inc.

Персоны

  • Frank L.A. (Institute of Biophysics, Siberian Branch, Russian Academy of Sciences)
  • Krasitskaya V.V. (Institute of Biophysics, Siberian Branch, Russian Academy of Sciences)

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