Тип публикации: статья из журнала

Год издания: 2024

Идентификатор DOI: 10.3390/ijms25020708

Аннотация: <jats:p>Gadolinium (Gd)-containing fullerenols are perspective agents for magnetic resonance imaging and cancer research. They combine the unique paramagnetic properties of Gd with solubility in water, low toxicity and antiradical activity of fullerenols. We compared the bioeffects of two Gd-containing fullerenols with a different Показать полностьюnumber of oxygen groups—20 and 42: Gd@C82O20H14 and Gd@C82O42H32. The bioluminescent bacteria-based assay was applied to monitor the toxicity of fullerenols, bioluminescence was applied as a signal physiological parameter, and bacterial enzyme-based assay was used to evaluate the fullerenol effects on enzymatic intracellular processes. Chemiluminescence luminol assay was applied to monitor the content of reactive oxygen species (ROS) in bacterial and enzymatic media. It was shown that Gd@C82O42H32 and Gd@C82O20H14 inhibited bacterial bioluminescence at >10−1 and >10−2 gL−1, respectively, revealing a lower toxicity of Gd@C82O42H32. Low-concentration (10−3–10−1 gL−1) bacterial bioluminescence activation by Gd@C82O42H32 was observed, while this activation was not found under exposure to Gd@C82O20H14. Additional carboxyl groups in the structure of Gd@C82O42H32 were determined by infrared spectroscopy and confirmed by quantum chemical calculations. The groups were supposed to endow Gd@C82O42H32 with higher penetration ability through the cellular membrane, activation ability, lower toxicity, balancing of the ROS content in the bacterial suspensions, and lower aggregation in aqueous media.</jats:p>

Журнал: International Journal of Molecular Sciences

Выпуск журнала: Т. 25, № 2

Номера страниц: 708

ISSN журнала: 16616596

Место издания: Basel

Издатель: Molecular Diversity Preservation International

• Stepin Evsei A. (Biophysics Department, School of Fundamental Biology and Biotechnology, Siberian Federal University, 660041 Krasnoyarsk, Russia)
• Sushko Ekaterina S. (Institute of Physics SB RAS, FRC KSC SB RAS, 660036 Krasnoyarsk, Russia)
• Vnukova Natalia G. (Department of Solid State Physics and Nanotechnology, School of Engineering Physics and Radioelectronics, Siberian Federal University, 660074 Krasnoyarsk, Russia)
• Churilov Grigoriy N. (Department of Solid State Physics and Nanotechnology, School of Engineering Physics and Radioelectronics, Siberian Federal University, 660074 Krasnoyarsk, Russia)
• Rogova Anastasia V. (Laboratory for Digital Controlled Drugs and Theranostics, FRC KSC SB RAS, 660036 Krasnoyarsk, Russia)
• Tomilin Felix N. (Laboratory for Digital Controlled Drugs and Theranostics, FRC KSC SB RAS, 660036 Krasnoyarsk, Russia)
• Kudryasheva Nadezhda S. (Institute of Biophysics SB RAS, FRC KSC SB RAS, 660036 Krasnoyarsk, Russia)

• Ядро РИНЦ (eLIBRARY.RU)