Тип публикации: статья из журнала
Год издания: 2022
Идентификатор DOI: 10.3390/catal12060643
Ключевые слова: butyrylcholinesterase, enzyme inhibition-based assay, enzyme stability, gelatin, immobilization, organophosphorus pesticides, starch
Аннотация: A new method of producing butyrylcholinesterase (BChE) preparations, stable in storage and use, has been proposed. The BChE preparation is the enzyme co-immobilized with 0.2 M 5-5′-dithiobis (2-nitrobenzoic acid) in starch or gelatin gel. All experimental preparations retain enzyme activity for at least 300 d. The preparations baseПоказать полностьюd on gelatin gel show higher activity but lower sensitivity to the toxicants tested in this study compared to the starch gel-based preparations. A method has been proposed for integrated detection of anti-cholinesterase substances in aqueous solutions using the experimental preparation with immobilized BChE. After the additional incubation of the preparation with the immobilized enzyme in the solution of the analyte, the detection limits of malathion and pirimiphos-methyl determined using the IC20 values were below their maximum allowable concentrations—0.005 µM and 0.03 µM, respectively. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
Журнал: Catalysts
Выпуск журнала: Vol. 12, Is. 6
Номера страниц: 643
ISSN журнала: 20734344
Издатель: MDPI